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ANSWER TO THE FOLLOWING QUESTION IN DETAIL
What is the basic principle of GPC (gel permeation chromatography)?
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- Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…Discuss in chemical detail the mechanism of action (how it works) of IMAC. Compare and contrast gel filtration chromotography with IMAC. Discuss the pros and cons for each technique. Why would a scientist prefer one technique over another? In what situations would one technique be more viable than the other?We’re back in the lab having fun! Our current experiment calls for us to treat our cells with THC (yeet!, delta 8 from hemp of course lol) and the final concentration of 15 µM once it has been added to the cells. We need to treat 10 mL of cells, and we don't want our treatment volume to be more than 10 µL per 1 mL of cells. What concentration should we make our stock solution? (I submitted this question before and got an answer of 150 µM and that was incorrect)
- Which of the following options shows the correct order of fastest to slowest motion of chloride ions, glycine and proteins through the separating gel? Chloride ions > Proteins > Glycine Glycine > Chloride ions > Proteins Chloride ions > Glycine > Protein Protein > Chloride ions > Glycine You are trying to separate a mixture of AMP, ADP, and ATP using ion exchange chromatography. Select appropriate supplies for your experiment. A positively charged column, deprotonated buffer, NaCl of same concentration. A positively charged column, protonated buffer, NaCl of increasing concentration. A negatively charged column, protonated buffer, NaCl of increasing concentration You cannot separate nucleotides using ion exchange chromatography.A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium it must be used immediately. Why?Which among the following demonstrates the difference between Native Polyacrylamide Gel Electrophoresis (PAGE) and SDS-PAGE? Group of answer choices Native PAGE has a denaturation step whereas SDS-PAGE does not have a denaturation step. Native PAGE is based on both the charge and size whereas SDS-PAGE is only based on size. Native PAGE does not have a denaturation step whereas SDS-PAGE has a denaturation step. Native PAGE is based only on charge whereas SDS-PAGE is based on both charge and size.
- We have 10 mL of cells to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 20 µM. How much of a 30 mM stock solution of H2O2 would we add to our cells? If you could please help me understand the steps of this problem!Explain how gel filtration chromatography works. What type of gel will you used when the protein size is 2500 Da? Explain.This is just a labster demonstration not a test, I am having trouble finding the answer in the text
- Gel-filtration chromatography separates molecules according to their size . Smaller molecules diffuse faster in solution than larger ones, yet smaller molecules migrate more slowly through a gel- filtration column than larger ones. explain this paradox. What should happen at very rapid flow rates?3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESWhich of the following technique is FALSE in microscopy? Osmium tetroxide can be used as a fixative as well as a negative stain in transmission electron microscopy. Two proteins; each tagged with their individual primary antibody followed by one protein with rhodamine conjugated secondary antibody and the other with fluorescein-conjugated secondary antibody can be visualized simultaneously using a fluorescence microscope. The emission spectrum of Rhodamine is 580 nm The emission spectrum of Fluorescein is 521 nm O An objective lens with 100X magnification and projection lens with 10X magnification is going to produce a 1000X total magnification. Phase contrast and differential interference contrast (DIC) do not require staining.