What does the brightness of a bond indicate in pcr? What does multiple bond appearance mean?

Q: What are the interesting characteristics of DNA quantitation? What are the positive and negative…

A: DNA quantification is done to know it's concentration and purity of the sample. This can be done…

Q: Amplified target regions of four different samples were separated using gel electrophoresis.  DNA…

A: Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA…

Q: Why do we need to use salt solution when we cloning dna?

A: Once the genomic deoxyribonucleic acid is certain to the silicon oxide membrane, the supermolecule…

Q: Which of the following pairs of sequences would be considered different alleles in DNA profiling?…

A: DNA profiling employs highly variable conserved regions known as variable number tandem repeats…

Q: Why is polyacrylamide gel is used in electrophoresis?

A: Introduction  Electrophoresis is a widely used laboratory technique for separating and analyzing…

Q: one should run the following

A: Pairwise sequence alignment is one form of a sequence alignment technique, where we compare only two…

Q: Refer to the attached DNA model.  In the spaces provided on the attached table, identify the…

A: DNA or deoxyribonucleic acid is the genetic material present in most organisms. It is composed of…

Q: a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y…

A: DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA…

Q: Why does DNA moves towards anode in gel electrophoresis?

A: Gel electrophoresis is process that separates the molecule usually protein or DNA on the basis of…

Q: Alcohol is considered to be a significant reagent for the isolation of nucleicacids (RNA and DNA).…

A: In molecular biology, the extraction of nucleic acids  DNA and RNA is the most crucial method. DNA,…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

A: 70% ethanol is used in washing DNA furing DNA extraction. 100% ethanol is used in the final step. In…

Q: What are the advantages of a Semi-solid dosage bond?

A: Semisolid dosage forms are a combination of solid and liquid used for local and systemic effects.…

Q: If a sample of double-stranded DNA gave the following results, what is the concentration (ng/uL) of…

A: The absorption value of 1 at A260 corresponds to 50 ug/ml So, the value of 1.637 at A260 corresponds…

Q: Q1) a) Draw the absorbance spectra for the following DNA samples. a. Pure DNA without protein…

A: “Since you have posted multiple questions with multiple sub parts, we will provide the solution only…

Q: DNA QUANTITATION Consult related references (textbooks or research articles) and list possible…

A: DNA quantitation refers to the process of measuring the amount of DNA present in a given sample.…

Q: samples Marker B 1,200 bp 1,000 bp 900 bp 800 bp 700 bp 600 bp s00 bp 400 bp 300 bp 200 bp 100 bp…

A: The given diagram is a representation of the process of gel electrophoresis. It is a laboratory…

Q: How is fluorescence in situ hybridization (FISH) used to producea spectral karyotype?

A: Fluorescence in situ hybridization (FISH) is a technique that is used to detect and locate a DNA…

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: In isolating DNA in strawberries, can you isolate other biomolecules aside from DNA just by using…

A: Biomolecules refer to the substances or molecules that are produced or secreted by biological…

Q: Why were 32P and 35S chosen for use in the Hershey–Chase experiment? Discuss the rationale and…

A: DNA (deoxyribonucleic acid) was discovered by Friedrich Miescher. Nucleotides are the structural…

Q: How can molecular structural analysis be accomplished with electrospray ionization mass…

A: Molecular structural analysis involves determining the arrangement and properties of atoms within a…

Q: Why are there different spec readings (OD600) for different strains of E. coli?

A: OD value means optical density value at 600nm wavelength of light. In spectroscopy the sample means…

Q: You do a PCR reaction,run a sample of the product on a gel,and use a dye to visualize the bands.…

A: Following changes can be done: 1. Since the upper band is non-specific, it is required to increase…

Q: What is “Autoradiography”? This method enables the determination of which macromolecule labeled with…

A: The techniques which provide images are useful in biological studies because they provide insight…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: Sanger sequencing method is chain terminating method.

Q: Based on the following image: A) Identify the largest DNA fragment in sample 5. Explain your…

A: In the given question, an agarose gel is shown which is having bands of DNA in different lanes. DNA…

Q: why are 2D NMR spectra symmetrical

A: 2D NMR spectra is used to determine the structure of a molecule. It is used for molecules that are…

Q: If the analysis of DNA from two different microorganismsdemonstrated very similar base compositions,…

A: The genetic material of bacteria and all the microorganisms is DNA which is comprised of nitrogenous…

Q: What is the biological and non-biological samples used in forensic DNA investigations?

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Each cell has…

Q: If you worked backward, starting with the amino acid sequence of the protein, would you obtain the…

A: The fundamental tenet of molecular biology is the idea that genetic material can only move from DNA…

Q: Describe the role of deoxyribonucleotides in generating DNA fragments for analysis?

A: DNA is hereditary material in most of the living organisms. DNA is long polymer of…

Q: In typing DNA from a sample found at a crime scene, how can a DNA mismatch prove that a suspect is…

A: DNA typing is also known as DNA fingerprinting. It is a method used to distinguish between…

Q: ) Poly A headdress and tail b) Ligase and helicase c) Amino acid and nitrogenous base

A: In eukaryotes, poly(A) tails usually act as stabilizers of intact mRNAs, whereas in E. coli they…

Q: Chargaff’s rules?

A: DNA's Base composition is governed by the relationships which are discovered by chargaff, known as…

Q: What are the benefits and disadvantages of IMAC for purifying proteins?

A: The purification of the proteins is performed by the use of different chromatographic procedures…

Q: Why does DNA appear when you add cold alcohol to the strawberry DNA solution?

A: