You do a PCR reaction,run a sample of the product on a gel,and use a dye to visualize the bands. Youexpect a single product of 300 bp, whichyou observe, but you also see a band ofabout 550 bp. Can you suggest a reasonwhy? Does the size of the unexpectedband indicate anything about your targetsequence?
You do a PCR reaction,run a sample of the product on a gel,and use a dye to visualize the bands. Youexpect a single product of 300 bp, whichyou observe, but you also see a band ofabout 550 bp. Can you suggest a reasonwhy? Does the size of the unexpectedband indicate anything about your targetsequence?
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter15: Genomes And Genomics
Section: Chapter Questions
Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...
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Question
You do a PCR reaction,
run a sample of the product on a gel,
and use a dye to visualize the bands. You
expect a single product of 300 bp, which
you observe, but you also see a band of
about 550 bp. Can you suggest a reason
why? Does the size of the unexpected
band indicate anything about your target
sequence?
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