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What are the molecular weights of the protein X polypeptides? (pick 1 only) - 50 kDa each - 60 kDa and 30 kDa - 70 kDa and 30 kDa What are the molecular weights of the protein Y polypeptides? (pick 1 only) - 60 kDa, 50 kDa, and 30 kDa - 50 kDa each - 60 kDa and 30 kDa

What are the molecular weights of the protein X polypeptides? (pick 1 only) - 50 kDa each - 60 kDa and 30 kDa - 70 kDa and 30 kDa What are the molecular weights of the protein Y polypeptides? (pick 1 only) - 60 kDa, 50 kDa, and 30 kDa - 50 kDa each - 60 kDa and 30 kDa

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Question 9. // Help solve the picture and the questions that were cut off from the picture attached. 

What are the molecular weights of the protein X polypeptides? (pick 1 only)
- 50 kDa each
- 60 kDa and 30 kDa
- 70 kDa and 30 kDa

What are the molecular weights of the protein Y polypeptides? (pick 1 only)
- 60 kDa, 50 kDa, and 30 kDa
- 50 kDa each
- 60 kDa and 30 kDa

A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. 1000 kDa 500 kDa 250 kDa 100 kDa 50 kDa 30 kDa 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa Native PAGE | || Non-reducing SDS-PAGE 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa Answer Bank Reducing SDS-PAGE
Transcribed Image Text:A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. 1000 kDa 500 kDa 250 kDa 100 kDa 50 kDa 30 kDa 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa Native PAGE | || Non-reducing SDS-PAGE 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa Answer Bank Reducing SDS-PAGE
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