Using one of the spectrophotometers in the lab, you measure a solution of unknown concentration and get an absorbance of 1.54 OD. You know the extinction coefficient is 0.49. What is the concentration of your solution? (Don't worry about the units in this example. Assume they are the same as what was used when determining the extinction coefficient.) 0.75 O2.08 0.32 3.14

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**Spectrophotometry Exercise: Determining Concentration**

Using one of the spectrophotometers in the lab, you measure a solution of unknown concentration and obtain an absorbance of 1.54 OD. You know the extinction coefficient is 0.49. What is the concentration of your solution? (Don't worry about the units in this example. Assume they are the same as what was used when determining the extinction coefficient.)

Choices:
- 0.75
- 2.08
- 0.32
- 3.14

**Instructions:** Calculate the concentration using the formula:

\[ \text{Concentration} = \frac{\text{Absorbance}}{\text{Extinction Coefficient}} \]

Plug in the given values to find the right concentration from the choices provided.
Transcribed Image Text:**Spectrophotometry Exercise: Determining Concentration** Using one of the spectrophotometers in the lab, you measure a solution of unknown concentration and obtain an absorbance of 1.54 OD. You know the extinction coefficient is 0.49. What is the concentration of your solution? (Don't worry about the units in this example. Assume they are the same as what was used when determining the extinction coefficient.) Choices: - 0.75 - 2.08 - 0.32 - 3.14 **Instructions:** Calculate the concentration using the formula: \[ \text{Concentration} = \frac{\text{Absorbance}}{\text{Extinction Coefficient}} \] Plug in the given values to find the right concentration from the choices provided.
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