Tubes 1-4 are standards with known mass and known volume of 200μL. Tubes 5-7 are unknowns that have been diluted 1/100 and volume used 200μL. How do you work out the concentration of tubes in g/L using a standard curve to work out the initial mass of unknowns.

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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Tubes 1-4 are standards with known mass and known
volume of 200µL. Tubes 5-7 are unknowns that have
been diluted 1/100 and volume used 200μL. How do
you work out the concentration of tubes in g/L using a
standard curve to work out the initial mass of unknowns.
Tube No.
1
2
3
4
5
6
7
Protein
amount
(ug)
0
50
100
200
Absorbanc
e (750nm)
0
0.32
0.5005
0.824
0.612
0.805
0.69
Transcribed Image Text:Tubes 1-4 are standards with known mass and known volume of 200µL. Tubes 5-7 are unknowns that have been diluted 1/100 and volume used 200μL. How do you work out the concentration of tubes in g/L using a standard curve to work out the initial mass of unknowns. Tube No. 1 2 3 4 5 6 7 Protein amount (ug) 0 50 100 200 Absorbanc e (750nm) 0 0.32 0.5005 0.824 0.612 0.805 0.69
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