Homework Help is Here – Start Your Trial Now!
Science
Chemistry
Tubes 1-4 are standards with known mass and known volume of 200μL. Tubes 5-7 are unknowns that have been diluted 1/100 and volume used 200μL. How do you work out the concentration of tubes in g/L using a standard curve to work out the initial mass of unknowns.

Tubes 1-4 are standards with known mass and known volume of 200μL. Tubes 5-7 are unknowns that have been diluted 1/100 and volume used 200μL. How do you work out the concentration of tubes in g/L using a standard curve to work out the initial mass of unknowns.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
See similar textbooks
icon
Related questions
Question
Tubes 1-4 are standards with known mass and known volume of 200µL. Tubes 5-7 are unknowns that have been diluted 1/100 and volume used 200µL. How do you work out the concentration of tubes in g/L using a standard curve to work out the initial mass of unknowns? | Tube No. | Protein amount (µg) | Absorbance (750nm) | |----------|--------------------|--------------------| | 1 | 0 | 0 | | 2 | 50 | 0.32 | | 3 | 100 | 0.5005 | | 4 | 200 | 0.824 | | 5 | | 0.612 | | 6 | | 0.805 | | 7 | | 0.69 | **Explanation:** The table above displays the relationship between the protein amount and the measured absorbance at 750nm for a series of tubes. Tubes 1-4 contain a known amount of protein, allowing for a standard curve to be established. By measuring the absorbance of tubes 5-7, which contain unknown concentrations but have been diluted, the initial concentration can be determined by referencing the standard curve. This involves plotting the known values to draw a curve and using the absorbance values of the unknowns to find their corresponding protein amount.
Transcribed Image Text:Tubes 1-4 are standards with known mass and known volume of 200µL. Tubes 5-7 are unknowns that have been diluted 1/100 and volume used 200µL. How do you work out the concentration of tubes in g/L using a standard curve to work out the initial mass of unknowns? | Tube No. | Protein amount (µg) | Absorbance (750nm) | |----------|--------------------|--------------------| | 1 | 0 | 0 | | 2 | 50 | 0.32 | | 3 | 100 | 0.5005 | | 4 | 200 | 0.824 | | 5 | | 0.612 | | 6 | | 0.805 | | 7 | | 0.69 | **Explanation:** The table above displays the relationship between the protein amount and the measured absorbance at 750nm for a series of tubes. Tubes 1-4 contain a known amount of protein, allowing for a standard curve to be established. By measuring the absorbance of tubes 5-7, which contain unknown concentrations but have been diluted, the initial concentration can be determined by referencing the standard curve. This involves plotting the known values to draw a curve and using the absorbance values of the unknowns to find their corresponding protein amount.
Expert Solution
Step 1: Interpret the problem statement

Given Data:

Chemistry homework question answer, step 1, image 1

To determine:

Initial mass of unknown in Test tube 5,6,7

steps

Step by step

Solved in 6 steps with 11 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Measurement
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
SEE MORE TEXTBOOKS