Toreterate, in your own words what is 1. The purpose of the selective factor in combination media? 2 The purpose of the differential factor combination media? Please use the text box below to provide your answers. *BIA E LE * EE 1 Paragraph

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Toreiterate, in your own words, what is:
1. The purpose of the selective factor in combination media?
2 The purpose of the differential factor combination media?
Piease use the text box below to provide your answers.
• Paragraph
MAC
MSA
The above image is for visual purposes only.
In the text box below, please indicate:
1. the selective and differential factors for MAC agar. You should use your lab manual to find this intormation.
2. the selective and differential factors for MSA You should use vour lab manual to find this information.
BIUA-A - I E E 3 * x, E E 12pt
Paragraph
Transcribed Image Text:Toreiterate, in your own words, what is: 1. The purpose of the selective factor in combination media? 2 The purpose of the differential factor combination media? Piease use the text box below to provide your answers. • Paragraph MAC MSA The above image is for visual purposes only. In the text box below, please indicate: 1. the selective and differential factors for MAC agar. You should use your lab manual to find this intormation. 2. the selective and differential factors for MSA You should use vour lab manual to find this information. BIUA-A - I E E 3 * x, E E 12pt Paragraph
(The top set of images are photographs of your results for MSA and MAC. The bottom set of images are illustrations that reflect the results you should have observed in the
photographs.)
Culture #:[Type here]
Organism:[Type here]
Name:
Combination Media:
Mystery Organism Identification
You will be given a pure culture of one of three organisms. Your assignment is to identify which of
the three possibilities is in your culture tube. To do this, you will use two different types of media:
Mannitol Salt Agar and MacConkey's Agar. Complete the table below by predicting the reactions
of each organism on each medium (Growth or no growth? If you expect growth, what color should
it be?). Do this before coming to lab on the due date. The information about each organism below
and the information found in the theory sections for the labs should help you make your
predictions. If you complete this table correctly, it will be a big help to you while you try to identify
MSA
MAC
your mystery organism!
E Submit this completed sheet on the assigned due date.
Organism
Appearance on
Mannitol Salt Agar
Appearance on
MacConkey's Agar
E. coli
(Type here]
[Type here]
P. mirabilis
[Type here)
[Type here)
MSA
S. aureus
MAC
[Type here]
[Type here)
Please record/explain what you observe on the agar plates ? and use the below table
Escherichia coli
E. coli is a Gram-negative facultative anaerobe. It is part of the normal gut flora of humans and
of the Combination Media to identify the unknown organism in your original
microtube. Which of the three organisms listed in the Combination Media matches the
growth patterns you observed on the MSA and MAC agar plates above?
other animals, where it helps in digestion and vitamin synthesis. As a member of the family
Enterobacteriacae, it exhibits both respiratory and fermentative metabolism. E. coli is capable of
fermenting several sugars including glucose, mannitol, lactose, sorbitol, arabinose, and maltose.
Proteus mirabilis
P. mirabilis is a Gram-negative facultative anaerobic rod. It is also a member of the family
Enterobacteriacae and is part of the normal gut flora in up to half of the human population. P.
mirabilis is not as diverse as E. coli in terms of the sugars it can utilize for fermentation (unlike E.
coli, P. mirabilis cannot ferment mannitol or lactose), it is able to hydrolyze urea, a capability that
E. coli does not possess.
Staphylococcus aureus
S. aureus is a Gram-positive, facultative anaerobic coccus. Although it is a potential pathogen, a
significant proportion of the general population (-20-30%) harbors S. aureus on the skin and nasal
mucosa, where it is able to live due to its osmotolerance (tolerance of high salt environments).
Substrates for fermentation include glucose and mannitol. S. aureus is distinguished from organisms
like Enterococcus faecalis by its production of catalase.
Record and explain the identity of the organism that received on Combination Media in
the top, right corner next to Organism.
Transcribed Image Text:(The top set of images are photographs of your results for MSA and MAC. The bottom set of images are illustrations that reflect the results you should have observed in the photographs.) Culture #:[Type here] Organism:[Type here] Name: Combination Media: Mystery Organism Identification You will be given a pure culture of one of three organisms. Your assignment is to identify which of the three possibilities is in your culture tube. To do this, you will use two different types of media: Mannitol Salt Agar and MacConkey's Agar. Complete the table below by predicting the reactions of each organism on each medium (Growth or no growth? If you expect growth, what color should it be?). Do this before coming to lab on the due date. The information about each organism below and the information found in the theory sections for the labs should help you make your predictions. If you complete this table correctly, it will be a big help to you while you try to identify MSA MAC your mystery organism! E Submit this completed sheet on the assigned due date. Organism Appearance on Mannitol Salt Agar Appearance on MacConkey's Agar E. coli (Type here] [Type here] P. mirabilis [Type here) [Type here) MSA S. aureus MAC [Type here] [Type here) Please record/explain what you observe on the agar plates ? and use the below table Escherichia coli E. coli is a Gram-negative facultative anaerobe. It is part of the normal gut flora of humans and of the Combination Media to identify the unknown organism in your original microtube. Which of the three organisms listed in the Combination Media matches the growth patterns you observed on the MSA and MAC agar plates above? other animals, where it helps in digestion and vitamin synthesis. As a member of the family Enterobacteriacae, it exhibits both respiratory and fermentative metabolism. E. coli is capable of fermenting several sugars including glucose, mannitol, lactose, sorbitol, arabinose, and maltose. Proteus mirabilis P. mirabilis is a Gram-negative facultative anaerobic rod. It is also a member of the family Enterobacteriacae and is part of the normal gut flora in up to half of the human population. P. mirabilis is not as diverse as E. coli in terms of the sugars it can utilize for fermentation (unlike E. coli, P. mirabilis cannot ferment mannitol or lactose), it is able to hydrolyze urea, a capability that E. coli does not possess. Staphylococcus aureus S. aureus is a Gram-positive, facultative anaerobic coccus. Although it is a potential pathogen, a significant proportion of the general population (-20-30%) harbors S. aureus on the skin and nasal mucosa, where it is able to live due to its osmotolerance (tolerance of high salt environments). Substrates for fermentation include glucose and mannitol. S. aureus is distinguished from organisms like Enterococcus faecalis by its production of catalase. Record and explain the identity of the organism that received on Combination Media in the top, right corner next to Organism.
Expert Solution
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In microbiology, one of the most important components is the medium. Microorganisms such as bacteria, fungus, and yeast can grow in it. It is used to isolate many types of bacteria in the laboratory.

 

 

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