To study the mechanism of SERCA, you prepare membrane vesicles containing this protein oriented such that its ATP binding site is on the outer surface of the vesicle. To measure pump activity, you use an assay that detects the formation of inorganic phosphate in the medium. When you add calcium and ATP to the medium, you observe phosphate production for only a short period of time. Only after the addition of calcimycin , a molecule that makes membranes selectively permeable to calcium, do you observe sustained phosphate production. Explain.
Cell Structure
The knowledge and concept about the structure of the cells have changed constantly over the past years. Primarily the scientist defines the cells as a simple membranous sac with fluids and some particles. Researches have now revealed that the cell is the more complex and basic unit of life. There are different types of cells and they also differ in shape and structure. The basic three parts of a cell are cell membrane, cytoplasm, and organelle.
Plant Cell
The smallest functional unit of a living organism is known as the cell. It is composed of cytoplasm enclosed by an outer layer known as the cell membrane. A cell is classified as a prokaryotic cell and a eukaryotic cell. A prokaryotic cell does not contain a nucleus, and the genetic material is freely suspended in the cell cytoplasm; for example, bacteria are composed of a prokaryotic cell. Whereas a eukaryotic cell contains a nucleus and membrane-bound cell organelles with a specific function, plants and animal cells are examples of eukaryotic cells.
Plant Cell Types
Plants are made up of a variety of cell types that include both living and dead cells. These cells provide them mechanical and structural support along with the growth of plants. Plant tissue is a group or collection of cells, which are similar in structure and work together to accomplish a specific function. Plant cells are most similar to animal cells, both being eukaryotic cells.
To study the mechanism of SERCA, you prepare membrane vesicles containing this protein oriented such that its ATP binding site is on the outer surface of the vesicle. To measure pump activity, you use an assay that detects the formation of inorganic phosphate in the medium. When you add calcium and ATP to the medium, you observe phosphate production for only a short period of time. Only after the addition of calcimycin , a molecule that makes membranes selectively permeable to calcium, do you observe sustained phosphate production. Explain.
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