To measure the blood Ca2+ concentration of a patient’s sample, 5.00 mL of blood was diluted to a total volume of 250.00 mL. A 10.00 mL portion of the diluted blood was further mixed with 40.00 mL of calcium dye solution and the final concentration of Ca2+ was determined to be 4.52 μM in the final solution. What’s the blood Ca2+ concentration for this patient

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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To measure the blood Ca2+ concentration of a patient’s sample, 5.00 mL of blood was diluted to a total volume of 250.00 mL. A 10.00 mL portion of the diluted blood was further mixed with 40.00 mL of calcium dye solution and the final concentration of Ca2+ was determined to be 4.52 μM in the final solution. What’s the blood Ca2+ concentration for this patient? (Assume that volumes are additive)

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Step 1: Introduction

To solve this question we have to careful about units and how many times successive dilution was done.

  1. 5 mL blood diluted to 250 mL.
  2. 10 mL taken and added to the 40 mL solution
  3. The total volume of the 4.52 μM solution is 50 mL.

μM = μmol/L = μmol/1000mL

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