2. Hemoglobin is a protein that carries oxygen in the blood. It absorbs light strongly at a wavelength of 400 nm, which gives blood its red color. A solution of hemoglobin is diluted to 10 times its original volume (1.0 mL hemoglobin + 9.0 mL water). The diluted solution has an absorbance value of 0.354. a) Determine the absorbance value of the original solution. Explain how you arrived at your answer. b) It would be simpler to measure the absorbance of the original solution directly. Explain why it is better to dilute the solution before measuring the absorbance for this sample. 3. Why is it important not to touch the sides of the cuvette with your fingers?

2. Hemoglobin is a protein that carries oxygen in the blood. It absorbs light strongly at a wavelength of 400 nm, which gives blood its red color. A solution of hemoglobin is diluted to 10 times its original volume (1.0 mL hemoglobin + 9.0 mL water). The diluted solution has an absorbance value of 0.354. a) Determine the absorbance value of the original solution. Explain how you arrived at your answer. b) It would be simpler to measure the absorbance of the original solution directly. Explain why it is better to dilute the solution before measuring the absorbance for this sample. 3. Why is it important not to touch the sides of the cuvette with your fingers?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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