The visible absorption spectrum for crystal violet, CV*, is shown in Figure 3. The concentration of the dye was 12.5 µM (12.5 x 106 M). What would be the optimum wavelength for generating a Beer's law calibration curve for crystal violet and measuring absorbance versus time for the reaction of CV* with OH? Explain your answer. Recall that absorbance measurements are most accurate and sensitive in the range 0.2-1.0.
The visible absorption spectrum for crystal violet, CV*, is shown in Figure 3. The concentration of the dye was 12.5 µM (12.5 x 106 M). What would be the optimum wavelength for generating a Beer's law calibration curve for crystal violet and measuring absorbance versus time for the reaction of CV* with OH? Explain your answer. Recall that absorbance measurements are most accurate and sensitive in the range 0.2-1.0.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Absorption Spectrum for Crystal Violet
Pre-Lab Questions:
1.8
1.6
1) The visible absorption spectrum for crystal violet, CV*, is
shown in Figure 3. The concentration of the dye was 12.5 µM
(12.5 x 10-5 M). What would be the optimum wavelength for
generating a Beer's law calibration curve for crystal violet
and measuring absorbance versus time for the reaction of
CV* with OH? Explain your answer. Recall that absorbance
measurements are most accurate and sensitive in the range
1.4
1.2
1
0.8
0.6
0.4
0.2
0.2–1.0.
350
400
450
500
550
600
650
700
750
Wavelength (nm)
Figure 3.
Absorbance
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