The visible absorption spectrum for crystal violet, CV*, is shown in Figure 3. The concentration of the dye was 12.5 µM (12.5 x 106 M). What would be the optimum wavelength for generating a Beer's law calibration curve for crystal violet and measuring absorbance versus time for the reaction of CV* with OH? Explain your answer. Recall that absorbance measurements are most accurate and sensitive in the range 0.2-1.0.

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Absorption Spectrum for Crystal Violet
Pre-Lab Questions:
1.8
1.6
1) The visible absorption spectrum for crystal violet, CV*, is
shown in Figure 3. The concentration of the dye was 12.5 µM
(12.5 x 10-5 M). What would be the optimum wavelength for
generating a Beer's law calibration curve for crystal violet
and measuring absorbance versus time for the reaction of
CV* with OH? Explain your answer. Recall that absorbance
measurements are most accurate and sensitive in the range
1.4
1.2
1
0.8
0.6
0.4
0.2
0.2–1.0.
350
400
450
500
550
600
650
700
750
Wavelength (nm)
Figure 3.
Absorbance
Transcribed Image Text:Absorption Spectrum for Crystal Violet Pre-Lab Questions: 1.8 1.6 1) The visible absorption spectrum for crystal violet, CV*, is shown in Figure 3. The concentration of the dye was 12.5 µM (12.5 x 10-5 M). What would be the optimum wavelength for generating a Beer's law calibration curve for crystal violet and measuring absorbance versus time for the reaction of CV* with OH? Explain your answer. Recall that absorbance measurements are most accurate and sensitive in the range 1.4 1.2 1 0.8 0.6 0.4 0.2 0.2–1.0. 350 400 450 500 550 600 650 700 750 Wavelength (nm) Figure 3. Absorbance
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