The table below describes a procedure in preparing standard solutions of a protein sample with different concentrations. (A) Determine the concentrations of each tube if the starting concentration of the protein is 500 mg mL. (B) Determine the equation of the line if the concentration (x-axis) was plotted vs the absorbance (y-axis). (C) What is the concentration, in mg mL, of the diluted unknown protein sample if the absorbance at 595 nm is 0.333? Tube Volume of protein, Volume of water, Final concentration, Absorbance # mL mL (at 595 nm) mg mL-1 1 0.00 5.00 0.113 2 0.70 4.30 0.184 3 1.40 3.60 0.251 14 2.10 2.90 0.329 2.80 2.20 0.385 6 3.50 1.50 0.454 7 4.20 0.80 0.503 5.00 0.00 0.577 Suppose that the unknown protein (diluted) sample in 4.C was diluted from a protein stock solution. Determine the concentration of the original unknown protein stock solution if 2.00 mL of the protein solution was mixed with 8.00 mL of water to create the unknown protein (diluted) sample in 4.C.
The table below describes a procedure in preparing standard solutions of a protein sample with different concentrations. (A) Determine the concentrations of each tube if the starting concentration of the protein is 500 mg mL. (B) Determine the equation of the line if the concentration (x-axis) was plotted vs the absorbance (y-axis). (C) What is the concentration, in mg mL, of the diluted unknown protein sample if the absorbance at 595 nm is 0.333? Tube Volume of protein, Volume of water, Final concentration, Absorbance # mL mL (at 595 nm) mg mL-1 1 0.00 5.00 0.113 2 0.70 4.30 0.184 3 1.40 3.60 0.251 14 2.10 2.90 0.329 2.80 2.20 0.385 6 3.50 1.50 0.454 7 4.20 0.80 0.503 5.00 0.00 0.577 Suppose that the unknown protein (diluted) sample in 4.C was diluted from a protein stock solution. Determine the concentration of the original unknown protein stock solution if 2.00 mL of the protein solution was mixed with 8.00 mL of water to create the unknown protein (diluted) sample in 4.C.
Chemistry & Chemical Reactivity
9th Edition
ISBN:9781133949640
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section4.8: Stoichiometry Of Reactions In Aqueous Solution-titrations
Problem 2RC
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