The rate of migration of nucleic acid fragments through an agarose gel is determined by the length of the agarose gel number of nucleotides in the fragment presence of hydrogen bonds between base pairs ratio of adenine to cytosine in the fragment volume of the starting sample
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
The rate of migration of
length of the agarose gel
number of
presence of hydrogen bonds between base pairs
ratio of adenine to cytosine in the fragment
volume of the starting sample
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