The pKa of MOPS is 7.20. Draw the equilibrium involved in the dissociation of the proton. 1. Suppose you dissolve 0.200 mol of MOPS in 1.00 Lof water. Calculate the pH of the solution. е. g. Use the pKa of MOPS to determine the ratio [A]/[HA] at pH 6.95. h. If you have 0.200 mol of MOPS dissolved in water, would you add NaOH or HCl to reach a pH of 6.95? And how many moles of the acid or base would you need to add? If you use a 100 mM MOPS buffer at pH 6.95 for a biochemical reaction which produces a maximum of 10 mM H*, which pH would you predict at the end of the reaction? i.

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he pka of MOPS is 7.20. Draw the equilibrium involved in the dissociation of the proton.
f.
Suppose you dissolve 0.200 mol of MOPS in 1.00 L of water. Calculate the pH of the
solution.
g.
Use the pKa of MOPS to determine the ratio [A]/[HA] at pH 6.95.
h. If you have 0.200 mol of MOPS dissolved in water, would you add NaOH or HCI to reach
a pH of 6.95? And how many moles of the acid or base would you need to add?
If you use a 100 mM MOPS buffer at pH 6.95 for a biochemical reaction which produces
a maximum of 10 mM H*, which pH would you predict at the end of the reaction?
i.
Transcribed Image Text:he pka of MOPS is 7.20. Draw the equilibrium involved in the dissociation of the proton. f. Suppose you dissolve 0.200 mol of MOPS in 1.00 L of water. Calculate the pH of the solution. g. Use the pKa of MOPS to determine the ratio [A]/[HA] at pH 6.95. h. If you have 0.200 mol of MOPS dissolved in water, would you add NaOH or HCI to reach a pH of 6.95? And how many moles of the acid or base would you need to add? If you use a 100 mM MOPS buffer at pH 6.95 for a biochemical reaction which produces a maximum of 10 mM H*, which pH would you predict at the end of the reaction? i.
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