f. Suppose you dissolve 0.200 mol of MOPS in 1.00 L of water. Calculate the pH of the solution. Use the pK, of MOPS to determine the ratio [A]/[HA] at pH 6.95. g. h. If you have 0.200 mol of MOPS dissolved in water, would you add NaOH or HCl to reach a pH of 6.95? And how many moles of the acid or base would you need to add? If you use a 100 mM MOPS buffer at pH 6.95 for a biochemical reaction which produces a maximum of 10 mM H*, which pH would you predict at the end of the reaction? i.

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f and g
f. Suppose you dissolve 0.200 mol of MOPS in 1.00L of water. Calculate the pH of the
solution.
Use the pk, of MOPS to determine the ratio [A]/[HA] at pH 6.95.
If you have 0.200 mol of MOPS dissolved in water, would you add NaOH or HCl to reach
a pH of 6.95? And how many moles of the acid or base would you need to add?
If you use a 100 mM MOPS buffer at pH 6.95 for a biochemical reaction which produces
a maximum of 10 mM H*, which pH would you predict at the end of the reaction?
g.
h.
i.
Transcribed Image Text:f. Suppose you dissolve 0.200 mol of MOPS in 1.00L of water. Calculate the pH of the solution. Use the pk, of MOPS to determine the ratio [A]/[HA] at pH 6.95. If you have 0.200 mol of MOPS dissolved in water, would you add NaOH or HCl to reach a pH of 6.95? And how many moles of the acid or base would you need to add? If you use a 100 mM MOPS buffer at pH 6.95 for a biochemical reaction which produces a maximum of 10 mM H*, which pH would you predict at the end of the reaction? g. h. i.
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