The O2-binding curve for Hemoglobin (Hb) is sigmoidal because: a   O2 binding to Hb increases the binding affinity of Hb for additional O2. b   The pH of blood is near the pKa of histidine. c   The pH of the bloodstream changes with changes in O2 concentration. d   Hb’s O2-binding affinity changes with changes in pH.

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