### Understanding the Role of the galX Gene in the Galactose Operon of E. coli#### OverviewThe inducible galactose operon of *E. coli* has three co-transcribed genes (galK, galE, galT) encoding essential enzymes: kinase, epimerase, and transferase, respectively. In this study, a regulatory mutant, galX\(^-\), has been isolated. The data table below summarizes the enzyme phenotypes in both the presence and absence of galactose for the wildtype and galX\(^-\) mutant strains. In this table, 'A' indicates the enzyme is absent, while 'P' indicates the enzyme is present.| Genotype | without galactose | with galactose ||------------|-------------------------------------|---------------------------------------|| | kinase | transferase | kinase | transferase || wildtype | A | A | P | P || galX\(^-\) | P | P | P | P |#### Determining the Role of the galX GeneTo elucidate the role of the galX gene, consider the phenotype of the galX\(^-\) mutant:- **What is the role of the galX gene?** - [Select] - **The phenotype of a galX\(^-\) mutant is an example of** - [Select]#### Experimental ApproachTo determine if the galX gene encodes a regulatory protein or serves as a regulatory site, a partial diploid (*galX\(^-\) galK\(^+\) galT\(^-\) / galX\(^+\) galK\(^-\) galT\(^+\)*) was created.#### Table for EvaluationAssign each number in blue (1-8) either 'A' or 'P' to indicate the enzyme phenotype expected if galX encodes a protein or serves as a regulatory site.| | without galactose | with galactose ||-----------------------|-------------------------------|--------------------------------|| | kinase | transferase | kinase | transferase || **as site** | **1** | **2** | **3** | **4** || **as protein** | **5** | **6** | **

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Description: ### Understanding the Role of the galX Gene in the Galactose Operon of E. coli#### OverviewThe inducible galactose operon of *E. coli* has three co-transcribed genes (galK, galE, galT) encoding essential enzymes: kinase, epimerase, and transferase,

Galactose operon is a prokaryotic operon involved in the utilization of galactose/galactose…

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The inducible galactose operon of E. coli has three co-transcribed genes (galk, galE, galT) encoding the kinase enzyme, epimerase enzyme, and transferase enzyme, respectively. You have isolated an interesting regulatory mutant, galX¯, with the phenotype shown in the second row of the following table. A means enzyme absent; P means enzyme present. without galactose with galactose genotype kinase transferase kinase transferase wildtype A A P P galx P P P P What is the role of the galX gene? [Select] The phenotype of a galX mutant is an example of [Select] To determine if the galX gene encodes a regulatory protein or serves as a regulatory site, you make a partial diploid with the following genotype: galX galK+ galT/ galX galK galT+ Complete the table below by assigning to each number in blue either A or P to indicate the enzyme phenotype expected if galX encodes a protein or serves as a site. without galactose with galactose kinase transferase kinase transferase as site 1 2 3 4 as protein 5 6 7 8 1 [Select] 2 [Select] 3 [ Select] 4 [Select] 5 [Select] 6 [Select] 7 [Select] 8 [Select]

The inducible galactose operon of E. coli has three co-transcribed genes (galk, galE, galT) encoding the kinase enzyme, epimerase enzyme, and transferase enzyme, respectively. You have isolated an interesting regulatory mutant, galX¯, with the phenotype shown in the second row of the following table. A means enzyme absent; P means enzyme present. without galactose with galactose genotype kinase transferase kinase transferase wildtype A A P P galx P P P P What is the role of the galX gene? [Select] The phenotype of a galX mutant is an example of [Select] To determine if the galX gene encodes a regulatory protein or serves as a regulatory site, you make a partial diploid with the following genotype: galX galK+ galT/ galX galK galT+ Complete the table below by assigning to each number in blue either A or P to indicate the enzyme phenotype expected if galX encodes a protein or serves as a site. without galactose with galactose kinase transferase kinase transferase as site 1 2 3 4 as protein 5 6 7 8 1 [Select] 2 [Select] 3 [ Select] 4 [Select] 5 [Select] 6 [Select] 7 [Select] 8 [Select]

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...

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