The image below presents the results of an experiment comparing the effect of Gentamicin and three concentrations of Acetic acid on *Escherichia coli*.Based on your knowledge about the disk diffusion method and the results of the experiment, what would be your recommendation on the use of Acetic acid to control *E. coli*?### Detailed Explanation of the Diagram:The diagram shows a petri dish with four disks placed on an agar plate inoculated with *Escherichia coli*. Each disk has a different label indicating the substance and concentration used:1. **1% Acetic Acid**: A disk labeled with 1% Acetic Acid. The inhibition zone around this disk is relatively small. 2. **2% Acetic Acid**: A disk labeled with 2% Acetic Acid. This disk shows a slightly larger inhibition zone than the 1% concentration.3. **5% Acetic Acid**: A disk labeled with 5% Acetic Acid. The inhibition zone around this disk is noticeably larger compared to the 1% and 2% acetic acid disks.4. **Gentamicin**: A disk labeled Gentamicin, showing the largest zone of inhibition among all disks, indicating high effectiveness against *E. coli*.The varying sizes of the inhibition zones around the disks demonstrate the effectiveness of each substance in controlling the bacterial growth of *E. coli*.

Disk Diffusion method or DDM is the method for antimicrobial substance susceptibility. The antimicrobial substance diffuses slowly from the reservoir in a nutrient agar plate inoculated with the microorganism. The absence of growth around the reservoir determines the strength or antimicrobial capacity of the substance being tested. if there is no growth, the antimicrobial substance is effective. Acetic acid is not recommended for control of E.coli growth. Gentamicin is an antibiotic and we can clearly see a zone of inhibition (ZOI) around gentamicin. The larger the size of ZOI formed, the higher the effect of the antimicrobial substance used. In comparison to gentamicin, the ZOI is not formed at 1% and 2%, hence these concentrations are not effective. When a 5% concentration of acetic acid is used, the ZOI is formed but of small size, hence making acetic acid less effective. Hence its use is not recommended.

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The image below presents the results of an experiment comparing the effect of Gentamicin and three concentrations of Acetic acid on Escherichia coli. Based on your knowledge about the disk Diffusion method, and the results of the experiment, what would be your recommendation on the use of Acetic acid to control E. coli? 1% acetic acid 5% acetic acid gentamicin 2% acetic acid

The image below presents the results of an experiment comparing the effect of Gentamicin and three concentrations of Acetic acid on Escherichia coli. Based on your knowledge about the disk Diffusion method, and the results of the experiment, what would be your recommendation on the use of Acetic acid to control E. coli? 1% acetic acid 5% acetic acid gentamicin 2% acetic acid

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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