The gelatin medium required inoculation with 3 loopfuls of culture. Why was so much organism required?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The gelatin medium required inoculation with
3 loopfuls of culture. Why was so much organism
required?
Gelatin is a large protein molecule that
requires a lot of gelatinase in order to
completely break down.
Like a flash mob these organisms only
produce gelatinase when their cell density hits
a certain threshold.
The gelatin in the medium can re-form after
breakdown therefore many cells were needed
to effectively destroy the protein.
The organisms needed to exhaust all other
nutrients before gelatinase would be
produced therefore a large amount of culture
is added to increase the rate of nutrient
breakdown.
Transcribed Image Text:The gelatin medium required inoculation with 3 loopfuls of culture. Why was so much organism required? Gelatin is a large protein molecule that requires a lot of gelatinase in order to completely break down. Like a flash mob these organisms only produce gelatinase when their cell density hits a certain threshold. The gelatin in the medium can re-form after breakdown therefore many cells were needed to effectively destroy the protein. The organisms needed to exhaust all other nutrients before gelatinase would be produced therefore a large amount of culture is added to increase the rate of nutrient breakdown.
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