The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular mass ( M, ). A newly purified protein (X) run on the same gel showed a single band that had moved a distance of 55 mm. What is the M, of protein X if the tracking dye covered a distance of 95 mm? Round off your answer to the nearest whole number. Protein M. Distance moved (mm) Transferrin 78 000 6.0 Bovine serum albumin 66 000 12.5 Ovalbumin 45 000 32.0 38.0 Glyceraldehyde-3- phosphate dehydrogenase carbonic anhydrase 36 000 29 000 50.0 Trypsinogen 24 000 54.0 Soyabean trypsin inhibitor 20 100 61.0 Myoglobin 17 800 69.0 Lysozyme 14 300 79.0 Cytochrome c 12 400 86.5

The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular mass ( M, ). A newly purified protein (X) run on the same gel showed a single band that had moved a distance of 55 mm. What is the M, of protein X if the tracking dye covered a distance of 95 mm? Round off your answer to the nearest whole number. Protein M. Distance moved (mm) Transferrin 78 000 6.0 Bovine serum albumin 66 000 12.5 Ovalbumin 45 000 32.0 38.0 Glyceraldehyde-3- phosphate dehydrogenase carbonic anhydrase 36 000 29 000 50.0 Trypsinogen 24 000 54.0 Soyabean trypsin inhibitor 20 100 61.0 Myoglobin 17 800 69.0 Lysozyme 14 300 79.0 Cytochrome c 12 400 86.5

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Of the following methods listed below, which could not be used to obtain a reasonable estimate of the molecular mass of a monomeric protein? Select all that apply. O Electrospray ionization mass spectrometrylsoelectric focusing O Gel filtration O All of these methods could be used O Isoelectric focusing O Sodium dodecyl sulfate polyacrylamide gel electrophoresis O Total amino acid sequence determination by Edman degradation
Norleucine (Nle) is a commonly used amino acid isostere for methionine (structure attached). Sketch a trace of an expected HPLC chromatogram of the tripeptide Ala-Gly-Nle after hydrolysis using a C18-reversed phase HPLC column.
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Question 30 Use the following chromatogram of standard amino acids: 24 11 16 19 12 21 15 23 13 14 17 18 20 22 1 12 14 16 Time (min) 8. 10 18 20 22 24 26 What is the estimated retention time of peak #4? 4 minutes 6 minutes 8 minutes cannot be determined from the given 2. (Au)
Select one answer for each question. For each question, response write a rationale for selection as supportive 1.An Analytical Chemist is analyzing a protein sample analysis using a light scattering technique. If the formulation of the subject sample has included surfactants or salt additives, which of the following would adequately apply? A.The additives would impact the ionic strength of the sample B.The impact of additives influence can be nullified when using a Light Scattering technique. C.The effect of formulation additives would impact the protein sample analysis and should be considered as a factor when analyzing the protein aggregation using the light scattering technique.
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A mixture of three proteins with pl = 7.5, 6.5, and 5.5 is separated using a DEAE-Sepharose column by eluting (washing) with buffer and NaCl. The column is eluted with aqueous buffer with a pH of 8.0, and an increasing [NaCl]. Which of the following proteins will elute from the column last? A The protein with pl = 5.5 B The protein with pl = 6.5 ☑ The protein with pl = 7.5 D These three proteins will not be separated, and will elute together. Your answer
4
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Number 4 please
2. The heptapeptide ALA-TYR-LYS-ASP-MET-CYS--SER was treated with trypsin and then with cyanogen bromide (CNBr). The pH of the resulting mixture was adjusted to a pH of 7.0 and chromatographed on an anionic exchanger column. Predict the order of the elution of the residual fragments. Draw each fragment with the correct net charge
Question 1