4 5. A scientist has a mixture of two peptides, A and B, and wants to separate them using ion exchangechromatography with a positively charged resin. The scientist has access to two different buffers with pHsof 4.5 and 10.SequenceplPeptide AN-term - GARNER - C-term9.6Peptide BN-term – YENSEN -C-term3.9a. Which buffer should the scientist use? Briefly describe your answer.b. Using the buffer you chose in part (a), which peptide will be eluted first? Briefly explain how youdetermined this.

(a) The resin is positively charged, i.e., anion-exchange resin. So, we need a buffer where both…

5. A scientist has a mixture of two peptides, A and B, and wants to separate them using ion exchange chromatography with a positively charged resin. The scientist has access to two different buffers with pHs of 4.5 and 10. Sequence pl N-term - GARNER - C-term N-term - YENSEN - C-term Peptide A 9.6 Peptide B 3.9 a. Which buffer should the scientist use? Briefly describe your answer. b. Using the buffer you chose in part (a), which peptide will be eluted first? Briefly explain how you determined this.

5. A scientist has a mixture of two peptides, A and B, and wants to separate them using ion exchange chromatography with a positively charged resin. The scientist has access to two different buffers with pHs of 4.5 and 10. Sequence pl N-term - GARNER - C-term N-term - YENSEN - C-term Peptide A 9.6 Peptide B 3.9 a. Which buffer should the scientist use? Briefly describe your answer. b. Using the buffer you chose in part (a), which peptide will be eluted first? Briefly explain how you determined this.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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