The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (μg) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 a. use excel, another program, or graph paper to produce a calibration curve based on the data. b. Use linear regression (best fit line) to obtain the slope of the line c. Determine the unknown protein content for a sample that shows an absorbance reading of 0.150.
The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (μg) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 a. use excel, another program, or graph paper to produce a calibration curve based on the data. b. Use linear regression (best fit line) to obtain the slope of the line c. Determine the unknown protein content for a sample that shows an absorbance reading of 0.150.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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The following data were obtained from a Bradford assay to determine the protein content in a sample:
| Protein content (μg) | Absorbance value |
| 0 | 0.000 |
| 2 | 0.022 |
| 5 | 0.065 |
| 10 | 0.106 |
| 20 | 0.178 |
| 30 | 0.299 |
| 40 | 0.380 |
| 50 | 0.472 |
| unknown | 0.150 |
a. use excel, another program, or graph paper to produce a calibration curve based on the data.
b. Use linear regression (best fit line) to obtain the slope of the line
c. Determine the unknown protein content for a sample that shows an absorbance reading of 0.150.
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