The Figure below shows the urease active site in the resting state (below left) and a very recent crystal structure of the active site with urea bonded (below right). In order to “trap" the urea adduct for scientific study, the cycle is blocked by replacing the hydroxo group by fluoride. Dashed lines represent hydrogen bonds. Lys (modified) Lys (modified) His His His His His His Он F His His His water water Asp Asp His water urea Figure 2 i) What is the function of the modified Lys bridge? i) Comment on mechanism proposed above in light of the urea-bound structure. i) How might the mechanism be revised?
The Figure below shows the urease active site in the resting state (below left) and a very recent crystal structure of the active site with urea bonded (below right). In order to “trap" the urea adduct for scientific study, the cycle is blocked by replacing the hydroxo group by fluoride. Dashed lines represent hydrogen bonds. Lys (modified) Lys (modified) His His His His His His Он F His His His water water Asp Asp His water urea Figure 2 i) What is the function of the modified Lys bridge? i) Comment on mechanism proposed above in light of the urea-bound structure. i) How might the mechanism be revised?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:The Figure below shows the urease active site in the resting state (below left)
and a very recent crystal structure of the active site with urea bonded (below
right). In order to "trap" the urea adduct for scientific study, the cycle is blocked
by replacing the hydroxo group by fluoride. Dashed lines represent hydrogen
bonds.
Lys
(modified)
Lys
(modified)
His
His
His
His
His
His
он
F
His
His
water
water Asp
Asp
His
His
water
urea
Figure 2
i)
What is the function of the modified Lys bridge?
ii)
Comment on mechanism proposed above in light of the urea-bound structure.
ii)
How might the mechanism be revised?
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