The ADNA genetic map (Figure 1), showing the positions of the important genes and thefunctions of the gene clusters.LysogenyDNA replicationCICRO CHOCIIRegulator oflate genesExcision/IntegrationProphage endLysisproteinJIKLMHTEFEDCBWAsVUcosTail genesFigtreThe DNA can be increased in size by only about 5%, representing the additionof only 3 kb of new DNA. You are developing a cloning vector based on ibacteriophage (Figure 1). Which segment of the genes can be removed from the DNAto increase the ability to carry a larger DNA fragment? Explain your reason.Head genesHow lambda DNA exists as a double stranded linear molecule during the infectioncycle?Spx su pe

Cloning vector Based on lambda bacteriophages: Two problems had to be solved before lambda based…

Answered: The DNA can be increased in size by…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

In a clinical context, a scientist is working with a viral DNA which is about 24000bps long. There are two known variants of the virus that share almost the same DNA but for a final fragment; with reference to Figure Q2b, the regions A and B are conserved in both variants, while the region C differs and is either 320bps (variant 1) or 380bps (variant 2). The scientist wants to set up a procedure to identify the variant they are dealing with. Viral dsDNA (i) (ii) (iii) Stable region (A) Variable region (C) Figure Q2b Known sequence (B) 5-GACCTCAATGTCCAGCGGTACGCTCATAAA-3' 3'-CTGGAGTTACAGGTCGCCATGCGAGTATTT-5' The scientists want to design a primer to amplify the variable region and to do so, they sequence a small fragment (sequence B) the conserved region close to the variable region C. Why is the scientist targeting a region outside of the fragment of interest? [3] The sequence of the fragment B is reported in Figure Q2b. Suggest a primer that can efficiently target this region and…
We transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp). Assume you were given competent cells of known transformation efficiency (TE). Assume TE= 1x10[7] (note 10[7] means 10 to the power of 7). You want to have about 1000 colonies on the P-200 plate. How many nanograms of plasmid should you use in the transformation reaction? Select only one answer. 1. 0.05 2. 20.00 3. 0.50 4. 50.00 5. 200.00 6. 5.00
A temperature sensitive mutant is isolated where at the non-permissive temperature replication stops relatively slowly and when the DNA is analyzed it appears to have extra twists ahead of the replication forks. What protein is most likely mutated? a)DnaA b)DNA polymerase I c)DNA gyrase d) DNA polymerase III
If deoxyribonucleotides that lack the 3’-OH groups are added during the replication process, what do you expect will occur? Describe what happens when a nonsense mutation is introduced into the gene encoding transposase within a transposon. A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?
In Oswald Avery experiment, two strains of pneumococcus bacteria were used to infect mice. Explain how the experiment discovered that DNA is the transformation agent.
Restriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…
Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.
For each of the following ( A & B ) provide the method of transfer and a brief explanation as to why the method would not take place under the conditions described . 1. Which method of DNA transfer between bacteria would not take place if the donor and recipient were separated by a filter with a pore size of 0.45 um or another physical barrier 2. Which method of transfer would be blocked by the presence of high concentrations of DNAase ( enzymes capable of degrading DNA ) ?
Chimeric DNA true are all except - a) Formed by linking DNA fragments of unrelated 2ood l v genome b) Sticky end producing restriction endonucleases favour formation of chimeric DNA c) They don't require DNA ligases d) The organism harbouring a chimeric DNA has features of themselves and the properties of the insert

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