The diagram below illustrates how recombinant DNA technology is used to produce valuable products. What is the purpose of using restriction enzymes in recombinant DNA technology? DF RISKIR DNA plasmids DO Bacterial cell (E. coli) Bacterium with recombinant DNA plasmid OO Replication of new gene OO Plasmids isolated OO Cloned cells produce new protein such as enzymes or hormones O-C Ligase joins sticky ends OO Restriction enzyme cuts plasmids Sticky ends Gene obtained from donor DNA using the same restriction enzyme O The restriction enzymes are used to catalyze the synthesis of new proteins such as enzymes or hormones. Sticky ends O The restriction enzymes are used to join sticky ends of both the cut plasmid and the cut gene obtained from donor DNA ma om

Q: During pcr primers can:  become graded by rnases be covslently linked to the template dtrand fall…

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene.

Q: If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the…

A: Primers are the short stretch of nucleotide sequences, which are used in a polymerase chain reaction…

Q: How is bacterial DNA safe from digestion by Restriction Endonucleases?     The plasmid of the…

A: Question-  How is bacterial DNA safe from digestion by Restriction Endonucleases?   A) The…

Q: Which is the correct order of the PCR reaction?

A: Introduction PCR or the polymerase Chain Reaction is a molecular technique used to amplify a…

Q: Plasmids are likely to contain

A: Plasmids are likely to contain:Antibiotic genes, A promoter associated with each gene, Restriction…

Q: Explain the role of each of the two enzymes used in the room temperature -PCR process.   Reverse…

A: PCR ( Polymerase Chain Reaction ) is a techniques which makes millions or billions copies of a DNA…

Q: Which of the options listed below can serve to carry a gene from one organism into a bacteria cell?…

A: The specific genes or pieces of DNA are transported or carried from one organism to another. These…

Q: Select any and all statements about polymerase chain reaction (PCR) that are true. There may be more…

A: Polymerase Chain Reaction (PCR) is an enzymatic process in which a specific region of DNA is…

Q: Primers are used in both PCR and in DNA sequencing. What is the purpose of the primers? O to…

A: The laboratory procedure known as PCR (polymerase chain reaction) is used to amplify (produce…

Q: When making recombinant DNA, why must you use the same restriction enzyme to cut the gene of…

A: The technique of creating multiple, identical copies of a specific portion of DNA is known as DNA…

Q: Pcr is an twchnique to amplify:   dna   rRna   mrna protein

A:

Q: Select the definition of a microsatellite. short pieces of DNA surrounding the periphery of the…

A: Answer

Q: By seeing the picture, how are Recombinant DNA formed? What is the difference between genetic…

A: Introduction: Genetic engineering or genetic modification or genetic manipulation involves direct…

Q: is used to seal the phosphodiester backbones between vector and insert. O DNA polymerase O Ligase…

A: Phosphodiester bonds are formed when exactly two of the hydroxyl groups in the phosphoric acid react…

Q: Pair the appropriate item with its purpose in the production of insulin a DNA containing the gene…

A:

Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

A: A plasmid is a double-stranded DNA molecule that is small and circular in shape.  Bactria and yeast…

Q: Restriction sites are palindromic, which means that they read the same in the 5′ to 3' direction on…

A: A palindrome sequence refers to a specific pattern of nucleotides that reads the same forward and…

Q: Pcr primers are:  single strand 15-25 bases long incorporated into the newly synthesized DNA…

A: PCR stands for polymerase chain reaction. It is one of the technique of biotechnology.

Q: Explain what restriction enzymes are, how they function and how they can be used to make recombinant…

A: Restriction enzymes are used to cut pieces of DNA.  Recombinant DNA is used in biotechnology to…

Q: Which of the following is not a component of Sanger sequencing? OddNTPs with fluorescent tags…

A: Question 1 explantion: The component not typically used in Sanger sequencing is restriction enzymes.…

Q: Which of the following DNA-synthesizing enzymes has 5' to 3' polymerization activity? Mark all the…

A: Polymerization is defined as the process in which tiny molecules known as monomers will chemically…

Q: Write the term or phrase that best completes each statement. Use these choices: gel electrophoresis…

A: PCR is biochemical research technique developed in 1985 by Kary Mullins. It is based on the…

Q: Many studies have linked plasmids to bacterial resistance to antibiotics.  Closured circular…

A: Focusing on plasmids as a target to combat antibiotic resistance holds promise but requires a…

Q: Why is DNA polymerase a required part of PCR? O It synthesizes new DNA O It synthesizes proteins O…

A: PCR is the abbreviation of Polymerase chain reaction. PCR is used to create millions of copies of…

Q: please answer these questions for this image What is the source of the DNA? What is the target?…

A: The objective of the question is to understand the process of genetic engineering, specifically the…

Q: In Polymerase Chain Reaction (PCR). Primers are short segments of DNA that. Obalance the pH of a…

A: Polymerase Chain Reaction or PCR is a molecular technique allowing the synthesis of multiple copies…

Q: Sticky ends are  1. DNA fragment with single - stranded ends 2. produced by the action of DNA…

A: DNA is the nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid in which…

Q: Consider the following:  Restriction enzymes bind and cut between bases T and A.  Based on this,…

A: VNTR  stands for Variable Number of Tandem Repeats, means the DNA sequence (Satellite DNA) repeats…

Q: If this gene is methylated, which of the following combinations of DNA fragments would you expect…

A: Answer: RESTRICTION ENZYME = It is the enzyme which cuts the strands of DNA at specific sites…

Q: MOLECULAR CLONING • Molecular cloning is the laboratory process used to create recombinant DNA. • It…

A: Recombinant DNA: it is a method of combining two distinct DNA (molecule) sequences, which may or may…

Q: The Centers for Disease Control and Prevention (CDC) program PulseNet uses genomic fragments that…

A: Pulsenet uses highly multiplexed amplicon sequencing (HMAs) panels for surveillance subtyping. It…

Q: PER product (Hbp 2 or Ap D) Puri Fied DNAS PETR gest Digested DNAS Ligate Plate e.cob HpD Transfurm…

A: Digestion of any DNA occurs by the exonucleases or endonucleases. The DNA ligases act as the…

Q: Which of the following components are required for the polymerase chain reaction (PCR)? Select all…

A: The components that are required for PCR are-  dNTPs, DNA polymerase, Oligonucleotide primers

Q: Which of the following statements is true of plasmids? A Agrobacteria use plasmids to induce tumors…

A: Options - 1, 2, 4, 5, 6  are true

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.