The Arp2/3 complex makes branched actin filaments by associating with a ‘mother’ actin filament and initiating assembly of a ‘daughter’ actin filament. Although it has been thought that Arp2/3 complex associates with and makes branches from the side of the ‘mother’ actin filament, it is alternatively possible that Arp2/3 complex associates with and makes branches from the barbed end of the ‘mother’ actin filament. Design an in vitro experiment (with purified proteins, such as actin and Arp2/3 complex) to determine which of these possibilities is correct by taking advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments. (1) Briefly describe your experiment in no more than three sentences (2) Draw diagrams of what the arrangement of actin filaments (simple lines are okay) are expected to look like in your proposed experimental and control conditions that indicate the possible outcomes of your experiment (3) Briefly describe your interpretation of the possible outcomes of your proposed experiment for the mechanism of Arp2/3 complex-mediated branch formation in no more than three sentences (4) Why are both Arp2/3 complex and capping protein good examples of quaternary protein structure
The Arp2/3 complex makes branched actin filaments by associating with a ‘mother’ actin filament and initiating assembly of a ‘daughter’ actin filament. Although it has been thought that Arp2/3 complex associates with and makes branches from the side of the ‘mother’ actin filament, it is alternatively possible that Arp2/3 complex associates with and makes branches from the barbed end of the ‘mother’ actin filament. Design an in vitro experiment (with purified proteins, such as actin and Arp2/3 complex) to determine which of these possibilities is correct by taking advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.
(1) Briefly describe your experiment in no more than three sentences
(2) Draw diagrams of what the arrangement of actin filaments (simple lines are okay) are expected to look like in your proposed experimental and control conditions that indicate the possible outcomes of your experiment
(3) Briefly describe your interpretation of the possible outcomes of your proposed experiment for the mechanism of Arp2/3 complex-mediated branch formation in no more than three sentences
(4) Why are both Arp2/3 complex and capping protein good examples of quaternary protein structure
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