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The approximate size (in kDa) of “Light Chain” is: ____   d. The approximate size (in kDa) of “Heavy Chain” is: ____

The approximate size (in kDa) of “Light Chain” is: ____   d. The approximate size (in kDa) of “Heavy Chain” is: ____

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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c. The approximate size (in kDa) of “Light Chain” is: ____

 

d. The approximate size (in kDa) of “Heavy Chain” is: ____

The image depicts a Western blot analysis, commonly used in molecular biology to detect specific proteins in a sample. **Description:** - **Molecular Weight Marker (Lane 1):** This lane contains a ladder of protein bands, indicating different molecular weights measured in kilodaltons (kDa). The weights are labeled on the side of the image as 188, 62, 49, 38, 28, 18, 14, 6, and 3 kDa. - **Sample Lane (Lane 2):** This lane shows bands corresponding to two protein components: - **Heavy Chain:** A band near the 49 kDa marker. - **Light Chain:** A band just below the 28 kDa marker. The bands correspond to the different chains of an IgG molecule, commonly studied in immunology. These chains are identified based on their size relative to known marker proteins. This type of analysis is key in studies involving protein identification, quantification, and validation in research related to cellular processes, disease mechanisms, and the development of therapeutic agents.
Transcribed Image Text:The image depicts a Western blot analysis, commonly used in molecular biology to detect specific proteins in a sample. **Description:** - **Molecular Weight Marker (Lane 1):** This lane contains a ladder of protein bands, indicating different molecular weights measured in kilodaltons (kDa). The weights are labeled on the side of the image as 188, 62, 49, 38, 28, 18, 14, 6, and 3 kDa. - **Sample Lane (Lane 2):** This lane shows bands corresponding to two protein components: - **Heavy Chain:** A band near the 49 kDa marker. - **Light Chain:** A band just below the 28 kDa marker. The bands correspond to the different chains of an IgG molecule, commonly studied in immunology. These chains are identified based on their size relative to known marker proteins. This type of analysis is key in studies involving protein identification, quantification, and validation in research related to cellular processes, disease mechanisms, and the development of therapeutic agents.
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Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. 

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