PROBLEM:The amino acid asparagine is synthesized fromaspartic acid by the enzyme asparagine synthetase(AS).In the previous problem you proposed a model forhow this gene could be regulated. Suppose that youcarry out an experiment to test your model. To dothis you cut out the regulatory sequences upstreamof the gene and fuse it to a gene for greenfluorescent protein (GFP). Now you can visuallyobserve when the gene is activated. You insert thisengineered gene into a host cell and look for GFPexpression. You discover some mutants that havedifferent expression levels of GFP and call themGFP1- and GFP2-. The expression levels of GFP aregiven below.CellGFP expressionWild type100GFP1-50GFP2- 11:15< ВackAssignment DetailsCell & Molecular Biology II-CRN#21490-202120given below.CellGFP expressionWild type100GFP1-50GFP2-Propose an explanation for these results based onyour model. In other words, what was mutated andhow?Your answer should include whether the mutation is(see links for more information):• dominant or recessivehttps://www.ncbi.nlm.nih.gov/books/NBK21578#A1877• in a cis regulatory element or trans regulatoryelementhttps://www.ncbi.nlm.nih.gov/books/NBK21635#A2540Submit Assignment( PreviousNext79CalendarTo DoNotificationsInbox

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The amino acid asparagine is synthesized from aspartic acid by the enzyme asparagine synthetase (AS). In the previous problem you proposed a model for how this gene could be regulated. Suppose that you carry out an experiment to test your model. To do this you cut out the regulatory sequences upstream of the gene and fuse it to a gene for green fluorescent protein (GFP). Now you can visually observe when the gene is activated. You insert this engineered gene into a host cell and look for GFP expression. You discover some mutants that have different expression levels of GFP and call them GFP1- and GFP2-. The expression levels of GFP are given below.

The amino acid asparagine is synthesized from aspartic acid by the enzyme asparagine synthetase (AS). In the previous problem you proposed a model for how this gene could be regulated. Suppose that you carry out an experiment to test your model. To do this you cut out the regulatory sequences upstream of the gene and fuse it to a gene for green fluorescent protein (GFP). Now you can visually observe when the gene is activated. You insert this engineered gene into a host cell and look for GFP expression. You discover some mutants that have different expression levels of GFP and call them GFP1- and GFP2-. The expression levels of GFP are given below.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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