Suppose that your Ph.D. mentor asks you to compare the kinetic behavior of the two recently discovered peptidase isozymes PepA and PepB. Your mentor's initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to distinguish the kinetic behaviors of PepA and PepB. All they had time to tell you was this: At low Tide5 concentration, the ?o (initial velocity) of PepA was greater than PepB, but at high Tide5 concentration, the reverse was true. Before you start your kinetic assays, you decide it would be wise to ponder this small bit of information. You have no numbers to work with, but does it tell you something about the relative values of ?m and ?max for PepA and PepB? Select the statements that are consistent with the information that you were given.

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Suppose that your Ph.D. mentor asks you to compare the kinetic behavior of the two recently discovered peptidase isozymes PepA and PepB. Your mentor's initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to distinguish the kinetic behaviors of PepA and PepB. All they had time to tell you was this: At low Tide5 concentration, the ?o (initial velocity) of PepA was greater than PepB, but at high Tide5 concentration, the reverse was true.

Before you start your kinetic assays, you decide it would be wise to ponder this small bit of information. You have no numbers to work with, but does it tell you something about the relative values of ?m and ?max for PepA and PepB?

Select the statements that are consistent with the information that you were given.
Suppose that your Ph.D. mentor asks you to compare the kinetic behavior of the two recently discovered peptidase isozymes
PepA and PepB. Your mentor's initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to
distinguish the kinetic behaviors of PepA and PepB. All they had time to tell you was this: At low Tide5 concentration, the V.
(initial velocity) of PepA was greater than PepB, but at high Tide5 concentration, the reverse was true.
Before you start your kinetic assays, you decide it would be wise to ponder this small bit of information. You have no numbers to
work with, but does it tell you something about the relative values of Km and Vmax for PepA and PepB?
Select the statements that are consistent with the information that you were given.
The Vmax of PepB is greater.
The Km of PepB is higher.
The Vmax of both isozymes could be the same, but the Km values are different.
The Km of both isozymes could be the same, but the Vmax Vvalues are different.
The ratio of Vmax /Km is larger for PepB.
Transcribed Image Text:Suppose that your Ph.D. mentor asks you to compare the kinetic behavior of the two recently discovered peptidase isozymes PepA and PepB. Your mentor's initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to distinguish the kinetic behaviors of PepA and PepB. All they had time to tell you was this: At low Tide5 concentration, the V. (initial velocity) of PepA was greater than PepB, but at high Tide5 concentration, the reverse was true. Before you start your kinetic assays, you decide it would be wise to ponder this small bit of information. You have no numbers to work with, but does it tell you something about the relative values of Km and Vmax for PepA and PepB? Select the statements that are consistent with the information that you were given. The Vmax of PepB is greater. The Km of PepB is higher. The Vmax of both isozymes could be the same, but the Km values are different. The Km of both isozymes could be the same, but the Vmax Vvalues are different. The ratio of Vmax /Km is larger for PepB.
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