Suppose that 6 micro moles (mM) of NADH were oxidized in the MDH reaction in two minutes. How many units of MDH were present?
Suppose that 6 micro moles (mM) of NADH were oxidized in the MDH reaction in two minutes. How many units of MDH were present?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Suppose that 6 micro moles (mM) of NADH were oxidized in the MDH reaction in two minutes. How many units of MDH were present?
Expert Solution
Step 1
The activity of enzymes is express in terms of units. and calculated using the micromoles of substrate transformed to product per minute. (1 Unit of the enzyme is defined by the amount required for the transformation of micromoles of substrate per minute.)
Enzyme activity = moles of substrate converted per unit time.
The enzyme Malate Dehydrogenase (MDH) rapidly oxidises the accumulated NADH.
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