Suppose a water sample with a known concentration of 100 coliforms/100 mL is tested using the multiple-tube fermentation method. Results show 5 positive tubes out of 10 in the 10 mL sample, 3 positive out of 10 in the 1 mL sample, and 1 positive out of 10 in the 0.1 mL sample. Calculate the MPN Index and discuss the potential sources of error in the MPN estimation process.

Suppose a water sample with a known concentration of 100 coliforms/100 mL is tested using the multiple-tube fermentation method. Results show 5 positive tubes out of 10 in the 10 mL sample, 3 positive out of 10 in the 1 mL sample, and 1 positive out of 10 in the 0.1 mL sample. Calculate the MPN Index and discuss the potential sources of error in the MPN estimation process.

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