student performs a separation by TLC on a silica get plate that results in three spots. A red spot is measured 23 mm away from the original sample spot, a blue spot is 42 mm away, and a yellow spot is 49 mm away. The distance from the original spot to where the solvent stopped is 55 mm. a) Calculate the Rf value for each component in the mixture. (Show your work and report the correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest to highest polarity. Explain your answer.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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A student performs a separation by TLC on a silica get plate that results in three spots. A
red spot is measured 23 mm away from the original sample spot, a blue spot is 42 mm
away, and a yellow spot is 49 mm away. The distance from the original spot to where the
solvent stopped is 55 mm.
a) Calculate the Rf value for each component in the mixture. (Show your work and report
the correct number of significant figures.)

b) The silica gel plate is polar. Rank the three components in the mixture from lowest to
highest polarity. Explain your answer.

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