Strain I Strain II Tet Amp C C Van P Tet tetracycline C=chromosome Van vancomycin P=plasmid = Amp = ampicillin I resistance 1. Strains I and II are plated on "confirmation" plates. Fill in the table with a + or - according to whether you think the bacteria will grown on the plate (+) or not (-)- Plain agar Agar + Amp Agar+Tet Agar+Van Agar + Van + Tet Agar+ Van + Amp Agar Tet+ Amp Strain I Strain II I 2. Strains I and II undergo conjugation and colonies from the "mating" plate are put on the following agar plates with antibiotics. Look at the results in the following table: Plain agar Agar + Amp Agar + Tet Agar + Van Agar + Van + Tet Agar+ Van + Amp Agar Tet+ Amp Strain III + ++ + a Which strain is the donor? b. Which strain is the recipient? C. d What genetic information was donated (plasmid, chromosome or both) Draw the genetic makeup of the recombinant Strain III

Strain I Strain II Tet Amp C C Van P Tet tetracycline C=chromosome Van vancomycin P=plasmid = Amp = ampicillin I resistance 1. Strains I and II are plated on "confirmation" plates. Fill in the table with a + or - according to whether you think the bacteria will grown on the plate (+) or not (-)- Plain agar Agar + Amp Agar+Tet Agar+Van Agar + Van + Tet Agar+ Van + Amp Agar Tet+ Amp Strain I Strain II I 2. Strains I and II undergo conjugation and colonies from the "mating" plate are put on the following agar plates with antibiotics. Look at the results in the following table: Plain agar Agar + Amp Agar + Tet Agar + Van Agar + Van + Tet Agar+ Van + Amp Agar Tet+ Amp Strain III + ++ + a Which strain is the donor? b. Which strain is the recipient? C. d What genetic information was donated (plasmid, chromosome or both) Draw the genetic makeup of the recombinant Strain III