Sphingosine 1-phosphate (SPP) is important for cell survival. The synthesis of SPP from sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. An understanding of the kinetics of the sphingosine kinase reaction may be important in the development of drugs to treat cancer. The velocity of the sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a stereoisomer of sphingosine that inhibits the enzyme. The results are shown below .[Sphingosine], μM 2.5 3.5 5 10 20 50 v0(mg·minꟷ1) no inhibitor 32.3 40 50.872 87.7 115.4 v0(mg·minꟷ1)with threo-sphingosine 8.5 11.5 14.6 25.4 43.9 70.8 Construct a Lineweaver-Burk plot to answer the following questions:(A)What are the apparent KMand Vmaxvalues in the presence and absence of the inhibitor?(B)What kind of inhibitor is threo-sphingosine? Explain this type of inhibition.

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