Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. DO NOT simply copy the dichotomous key from you lab manual or from other sources (e.g. Bergey’s manual). The organisms are not the same as the ones used in this exercise. Also, because of genetic variability between organisms, the results may not be same as in our lab book or in other sources. Use the tables in your lab notes to create your key. The key must include a source for each test. You can use footnotes in your key or just include the information right next to test name. Since the tests we use come from either the lab manual or the Lab Notes, reference each test by citing the exercise number or the Lab Notes. Example: "6.5% NaCl Broth, Exam 3 Lab Notes page 6" or "Nitrate Reduction Test, Ex. 5-7". 4. Your dichotomous key must be typed. If you need more room than one page, feel free to use two. You can use Powerpoint, Word, or the Google equivalents to type up your key. Use text boxes and connectors to make a flow chart. Also, there is a cool site called Creately (Links to an external site.) that allows you to make dichotomous keys. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester.
- You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the
Gram Stain . Subsequent steps will include biochemical tests only. DO NOT simply copy the dichotomous key from you lab manual or from other sources (e.g. Bergey’s manual). The organisms are not the same as the ones used in this exercise. Also, because of genetic variability between organisms, the results may not be same as in our lab book or in other sources. Use the tables in your lab notes to create your key. - The key must include a source for each test. You can use footnotes in your key or just include the information right next to test name. Since the tests we use come from either the lab manual or the Lab Notes, reference each test by citing the exercise number or the Lab Notes. Example: "6.5% NaCl Broth, Exam 3 Lab Notes page 6" or "Nitrate Reduction Test, Ex. 5-7". 4.
- Your dichotomous key must be typed. If you need more room than one page, feel free to use two. You can use Powerpoint, Word, or the Google equivalents to type up your key. Use text boxes and connectors to make a flow chart. Also, there is a cool site called Creately (Links to an external site.) that allows you to make dichotomous keys.
Possible Organisms
- Alcaligenes faecalis
- Enterobacter aerogenes
- Enterococcus faecalis
- Escherichia coli
- Proteus vulgaris
- Pseudomonas aeruginosa
- Salmonella arizoniae
- Staphylococcus aureus
- Staphylococcus epidermidis
- Staphylococcus saprophyticus
- Streptococcusbovis
- Streptococcus pyogenes
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