Sediment Stains I. IDENTIFICATION: Identify the correct sediment stains used. Choose the answer from the box below. ________________1. It delineates structure and contrasting colors of the nucleus & cytoplasm. Identified WBC’s, epithelial cells & casts. ________________2. Enhances nuclear detail; supravital stain. Differentiates WBCs and RTE cells. ________________3. Lyses RBCs, enhances nuclei of WBCs. Distinguishes RBCs from WBCs, yeast, oil droplets & crystals. ________________4. Stains triglycerides and neutral fats orange-red. Identifies free fat droplets & lipid-containing cells & casts. ________________5. Differentiates Gram positive * negative bacteria. Identifies bacterial casts. ________________6. Stains eosinophilic granules. Identifies urinary eosinophils. ________________7. Stains structures containing iron. Identifies hemosiderin granules in cells & casts. Prussian Blue 2% Acetic Acid Gram Stain Sternheimer-Malbin Stain Hansel Stain (Eosin Y + Methylene Blue) Oil Red O and Sudan III Toluidine Blue

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