Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
RESTRICTION ENZYME MAPPING
Predict the result

Transcribed Image Text:PGLO digest
Volume to add
Tube 1
Tube 2
Mass or Conc.
Tube 3
Tube 4
Component
Concentration
(negative
control)
(EcoRI,
HindlII)
(HindllI)
required
(EcoRI)
DNA
unknown
1 pg
Universal
1X
buffer
10X
Water
EcoRI
0.5 units/ul.
1 unit
none
Hind III
1 unit
none
0.5 units/ul
20 μL
20 μL
20 μL
20 μL
ТОTAL
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