Q: What are the 5 gene in order on the E. coli tryptophan operon?
A: The trp operon, found in the bacterium Escheichia coli is a group of genes that encode biosynthetic…
Q: Explain and give examples of Inducible & Repressible Operons. Describe what types of protein…
A: Inducer: Inducer is the substance that causes the gene for protein synthesis to be activated.…
Q: Name and describe two types of constitutive mutants of the lactose operon.
A: Operon can be defined as an operating unit, which is nothing but gene clusters that are located…
Q: Why is step 2 correct, is it because this is a repressible operon?
A: Operon is a cluster of genes which contains operator, promoter and structural genes. It is a kind of…
Q: Define an operon.give an example,exaplain an inducible operon?
A: Gene regulation is the mechanism which acts to either induce or repress the gene expression. It…
Q: Describe the main features of repressible operons.
A: An operon is defined as a particular set of genes present on a DNA that operates under same…
Q: If you add a co-eepressor to a repressor protein and that allows it to bind the operator is this a…
A: Inducible operon is one that is expressed in the presence of inducer. Constitutive operon is the…
Q: Utilizing lactose operon, how can we explain how induction controls enzyme synthesis 2. Use…
A: An operon is a group of genes that code for proteins that are involved in a single cellular…
Q: 2. Distinguish among inducible, repressible, and constitutive gene operons.
A: An operon is a functional unit of genomic DNA that comprises a collection of genes that are all…
Q: The trp operon in E. coli is a negative repressible operon. This implies that... a. The trp…
A: The correct answer is:c. The trp operon makes use of a repressor and can be turned "on" (i.e., is…
Q: 12. You want to determine the location of the promoter and operator for an operon. To do so, you…
A: 1) If operator is removed, expression becomes constitutive 2) If promoter is removed, there should…
Q: How does induction control enzyme synthesis using lactose operon? 2. Describe how level of a key…
A: By controlling the transcription of the mRNA which is needed for the enzyme synthesis, the genetic…
Q: Gene expression in bacteria is controlled by the operon model. a) Why is the operon model…
A: BASIC INFORMATION OPERON MODEL This model of gene regulation was put forward by Francis Jacob and…
Q: What do you mean by coordinate expression of genes in operon?
A: Answer- In prokaryotes genes are controlled by one promoter region that is called polycistronic…
Q: what is an attenuator? (in context of a prokaryotic operon )
A: Operons are prokaryotic arrangements of multiple genes under the control of a single promoter. The…
Q: Which of the following is false when the genes of the Lac operon are being tran- scribed in high…
A: Lac operon is a set of genes used by E.coli to metabolize lactose when glucose is not available for…
Q: Consider the lac operon of E. coli. Specifically, explain the following: a. Describe the overall…
A: The lac operon is a collection of genes located in the chromosome of Escherichia coli, and it…
Q: According to the operon model, for the synthesis of an inducible enzyme to occur, the end-product…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: Compare and contrast the functional mechanism of the Inducible - Lac operon with the Repressible -…
A: Gene expression is a process in which the genetic instructions of genes are utilized to manage…
Q: Which of the following lac operon genotypes would allow for functional versions of all the…
A: lac operon: - Inducible system - Both positive and negative regulation. - Involved in lactose…
Q: Define the term operon and explain one advantage it provides to a bacterial cell
A: The cells are the primary unit of life. Based on the number of cells an organism may be prokaryotic…
Q: Describe the difference between a repressible and an inducible operon.
A: The theory of Operon comes from the concept of "gene-regulation" proposed by Jacob and Monod. It is…
Q: 3. Draw an anabolic operon with three biosynthetic genes for compound Q and a catabolic operon with…
A:
Q: What is an operon? Explain an inducible operon.
A: In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control…
Q: Distinguish between a repressible operon and aninducible operon.
A: The Operon is the regulatory system present in DNA in cluster form, which contains structural genes…
Q: 5. Use the table below to describe under what conditions the lac operator is turned on and off. Is…
A: The lac operon is a single-promoter operon that is necessary for the transport and metabolism of…
Q: Differentiate inducsible and repressible operon
A: The promoter and operator control the group of genes which are called operons. Some operons are…
Q: Describe the feature of lac operon?
A: The operon comprises the genes regulating a metabolic reaction. It is also an operon. It mainly…
Q: Compare the types of bacterial genes associated with inducible operons, those associated with…
A: Gene regulation is a collection of mechanisms that the cell employs to reduce or increase the…
Q: What is the major difference between the mechanism involved inattenuation and riboswitches and the…
A: Attenuation is a mechanism that controls gene expression in a bacterial operon and causes premature…
Q: What is a negative inducible operon?
A: Regulated genes can be switched on/off depending on cell's metabolic needs. Operon It is a group of…
Q: Describe the main features of the lactose operon and inducibleoperons.
A: Inducible operons are those in which genes for certain enzymes can be turned on by the substrate of…
Q: Does it need active form or inactive form of the repressor ? 3) What is role of RNA polymerase,…
A: An operon is defined as the collection of coordinately regulated genes . Q2Ans:In a negative control…
Q: What is the phenotype of an E. coli strain with a mutation in the lac operator that allows the lac…
A: Operon is defined as a region of DNA where it is made up of clusters of related genes arranged under…
Q: Determine the outcome for the lac operon genotype shown below: I*p*o°z*y /I*p*o*z°x* Assume lactose…
A: A group of genes that are transcribed under a single promoter and an operator is referred to as…
Q: How does cAMP and allolactose work to influence the operon?
A: The two proteins involved regulation of lac operon are CRP (catabolite repressor protein) and lac…
Q: I. Answer the following: 1. What are the main elements of the lac operon and their functions? 2.…
A: Definition:- An operon is a group of genes that are transcribed at the same time. These are a…
Q: What advantages are there to having genes arranged in an operon, compared with the arrangement in…
A: The gene is the basic morphological and physiological unit of heredity. DNA is used to make genes.…
Q: Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein.…
A: The operon model is applicable in bacterial gene regulation and also applicable in some lower…
Q: Suppose you have an E. coli in which the gene that codes for the lac repressor protein has been…
A: Lac operon is an inducible type of operon that involves the breakdown of lactose in E.coli. The lac…
Q: When would the lac operon in E. coli be repressed, and why?
A: Operon includes a group of structural genes and regulatory sequences.
Q: Can you think for how long the lack operon would be expreesed in the presence of lactose?
A: Lac operon is an operon that regulates the breakdown of lactose present in the medium. It contains…
26 .
What is the difference between a repressible and an inducible operon?
Step by step
Solved in 3 steps
- For each of the following conditions in the Lac Operon, state whether transcription will occur or not and state why. Include all the major actors including glucose, lactose, cAMP and CAP(CPR). Key + (present) - (absent) 1) Glucose-; Lactose- 2) Glucose+; Lactose- 3) Glucose+; Lactose+ 4) Glucose-; Lactose+What is the relation between Blue/ White Screening and lac operon?If a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would you expect? A) The three genes of the lac operon will be expressed normally. B) The repressor will not be able to bind to the operon. C) The operon will still transcribe the lacZ and lacY genes, but the mRNA will not be translated. D) LacZ will not be transcribed and Beta galactosidase will not be produced.
- Strain ROFL4 has a premature stop mutation in the lacZ gene, resulting in a nonfunctional b-galactosidase. Otherwise all other parts of the operon are functional. 1. Can strain ROFL4 metabolize lactose if it is present? 2. If lactose is absent, will strain ROFL4 transcribe its lac operon? 3. If lactose is present, will strain ROFL4 transcribe its lac operon?A researcher was trying to determine whether two molecules(A nd B) were corepressors or inducers in their respective operon systems. Data was collected regarding the levels of protein and the amount of gene transcription for the genes in theri respectiver operons. The data is shown below. Level of protein Transcription of gene 1 low Transcription of gene 2 Molecule A High low Low high high Molecule B High high high Low low lowThe organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…
- Operator | Choose [Choose) A set of genes transcribed together under the regulatory control of a singe promoter A regulatory protein that binds to an region of non-coding DNAgene that suppresses transcriptionby blocking RNA polmer A segment of DNA where the repressor binds to, therety preventing the transcription of certain genes An operon that isalways "off" - turned on by environmental cues an operon that isalways "on"- turned off by environmental cues TChoose Operon Repressor Repressible Operon [ Choose nducible operon [Choose |Decide which operon each of the following characteristics applies to. Note: a description may apply to both. a. lac operon b. trp operon 68. repressible 69. Turned off when end product is present (feedback inhibition) 70. Promoter, activator region, and 3 genes 71. Repressible 72. contain operator and promoter regions as well as transcript units of at least two or more coding genes 73. Codes for catabolic enzymes that break down lactose 74. Inducible 75. Promoter, activator region, and 5 genes 76. Codes for anabolic enzymes that help manufacture tryptophan 77. Lactose is the co-inducer 78. Turned on when substrate is present 79. Tryptophan is the co-repressor 80. Regulatory gene segmentsThis figure represents the ABC operon, which is a negative inducible operon, and its associated regulatory gene. The structural genes produced by the operon convert molecule A into molecule D. When molecule A is present in the environment, it will bind to the regulatory protein, and operon expression will be induced. operator testR testR promoter regulatory protein testP testo structural genes test1 test1 test2 test2 test3 test3 structural proteins
- 3 5. Suppose you performed a B-galactosidase assay on E. coli with a l*P+O°Z*Y operon. What results would you expect in the absence and presence of IPTG? 6. If you did not get the expected results for the previous question, but you knew the genotype of the operon was correct, name two ways your experiment could have gone wrong.1. Compare and contrast repressible and inducible operons. 2. Are genes for enzymes involved in anabolic processes usually repressible or inducible , why?28. Which statement does NOT explain why the lac operon would not be effectively regulated by an attenuation mechanism like the trp operon? 1.Lactose is a substrate for the lac operon protein products. 2.Products of lac operon enzymes are not used in protein synthesis. 3.Transcription and translation do not happen simultaneously for the lac operon. 4.Attenuation is a suitable regulatory mechanism for anabolic amino acid pathways. 5.All of these statements explain why regulation by attenuation would not be effective for the lac operon.