b) Briefly explain what happens to vo, rate of product formation, when it is affected by an allosteric inhibitor. This conformation of the enzyme active site is known as -state. c) True/False. Allosteric regulators alter the Vmax of an allosteric enzyme. Question 4. a) Briefly explain what happens to vo, rate of product formation, when it is affected by an allosteric activator. This conformation of the enzyme active site is known as _ -state. V₁ = kcat [ES]

b) Briefly explain what happens to vo, rate of product formation, when it is affected by an allosteric inhibitor. This conformation of the enzyme active site is known as -state. c) True/False. Allosteric regulators alter the Vmax of an allosteric enzyme. Question 4. a) Briefly explain what happens to vo, rate of product formation, when it is affected by an allosteric activator. This conformation of the enzyme active site is known as _ -state. V₁ = kcat [ES]

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Some enzymes can be regulated by covalent modification, in which a group is covalently bonded to an amino acid side chain. Phosphorylation of side chains is a common regulatory covalent modification. In this essay, you will explore phosphorylation of side chains. Compare and contrast the types of interactions a free alcohol side chain such as serine could make with that of a phosphorylated alcohol such as phosphoserine (pSer). Could this modification affect the 3D structure of the protein? How? Imagine you are trying to separate a protein containing an unphosphorylated S residue from the same protein containing a pSer residue. Discuss how you could use ion-exchange chromatography to separate these two proteins
The natural enzyme is recreated by mixing isolated regulatory and catalytic subunits of ATCase. What biological relevance does this observation have?
In a typical enzyme-catalyzed reaction, what are the relative concentrations of reactants and products as compared to the enzyme concentration?
A potent inhibitor effectively inhibits an enzyme catalyzed reaction. What kind of a Ki value you would expect for a potent inhibitor?
In bacteria, isocitrate dehydrogenase is regulated by phosphorylation of a specific Ser residue in the enzyme active site. X-ray structures of the phosphorylated and the nonphosphorylated enzyme show no significant conformational differences. How does phosphorylation regulate isocitrate dehydrogenase activity? O The phosphoryl group sterically hinders the substrate. O The phosphorylation bears a negative charge, which repels the substrate. O The phosphoryl group attracts positively charged Ca2* cations, which block the active site on the enzyme. None of the above.
Although we have introduced catalytic strategies separately, an enzyme typically employs more than one during a reaction. The catalytic strategies employed by serine protease include: Select one or more: O a. reduction of ASt b. transition state stabilization Oc. metal-assisted catalysis O d. acid/base catalysis O e. covalent catalysis
3) Read the situations below and indicate which of the four methods of enzyme regulation is occurring for each. a) The energy-carrying molecule ATP is made by the enzyme ATP synthase. Muscle cells use a lot of energy and also have higher amounts of the ATP synthase enzyme than many other cell types. General mechanism of enzyme regulation: S b) Prostaglandins are messenger molecules involved in the inflammatory response, as well as the perception of pain. They are synthesized from polyunsaturated fatty acid substrates by an enzym called cyclo-oxygenase. "Ibuprofen" is the active ingredient in a variety of anti-inflammatory medications such as Motrin® and Advil®. It reduces pain and swelling by binding to a hydrophobic channel in the active site of cyclo-oxygenase, blocking the polyunsaturated fatty acids from binding to the enzyme, and therefore stopping production of prostaglandins. General mechanism of enzyme regulation:
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