Question 26 A solution of nicotinamide adenine dinucleotide (NADH, MM=663.43 g/mol) was analyzed by fluorescence. 10.77 mL of the NADH unknown is diluted to 46.43 mL and a fluorescence signal of 26.8 was measured. 1.20 mmol of a NADH standard is added to 10.77 mL of the NADH unknown and diluted to 46.43 mL. A fluorescence signal of 70.6 was measured. Calculate the concentration of NADH in the unknown in units of mmol/L (mM). Report your answer to one decimal place.

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Question 26
A solution of nicotinamide adenine dinucleotide (NADH, MM=663.43 g/mol) was analyzed by
fluorescence.
10.77 mL of the NADH unknown is diluted to 46.43 mL and a fluorescence signal of 26.8 was
measured.
1.20 mmol of a NADH standard is added to 10.77 mL of the NADH unknown and diluted to
46.43 mL. A fluorescence signal of 70.6 was measured.
Calculate the concentration of NADH in the unknown in units of mmol/L (mM).
Report your answer to one decimal place.
Transcribed Image Text:Question 26 A solution of nicotinamide adenine dinucleotide (NADH, MM=663.43 g/mol) was analyzed by fluorescence. 10.77 mL of the NADH unknown is diluted to 46.43 mL and a fluorescence signal of 26.8 was measured. 1.20 mmol of a NADH standard is added to 10.77 mL of the NADH unknown and diluted to 46.43 mL. A fluorescence signal of 70.6 was measured. Calculate the concentration of NADH in the unknown in units of mmol/L (mM). Report your answer to one decimal place.
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