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QUESTION 2 An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units per minute. Given that the molar absorption coefficient (e) is 6220 M1 cm1 and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in μmol per minute per mL? a. 8.04x10-8 b. 80.4 C. 8.04x10-5 d. 0.0804 QUESTION 3 A cell lysis buffer is made up with a pH of 7.9. What is the concentration of hydrogen ions in the buffer? a. 7.94 x 107 M b.3.71 x 10-4M C. 0.89M d. 1.26 x 10-8M

QUESTION 2 An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units per minute. Given that the molar absorption coefficient (e) is 6220 M1 cm1 and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in μmol per minute per mL? a. 8.04x10-8 b. 80.4 C. 8.04x10-5 d. 0.0804 QUESTION 3 A cell lysis buffer is made up with a pH of 7.9. What is the concentration of hydrogen ions in the buffer? a. 7.94 x 107 M b.3.71 x 10-4M C. 0.89M d. 1.26 x 10-8M

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Bb Take Test: Practical report 2 X C Apps LINDE Secure https://blackboard.le.ac.uk/webapps/assessment/take/launch.jsp?course_assessment_id=_39582_1&course_id=_41417_1&content_id=_ Unavailable in you... Amazing Opportu... Palmar Oceanfron...lv Bag, Medical Dr... JD Fila Disruptor II R... 3. Edexcel Revisio... Edexcel GC Question Completion Status: QUESTION 2 -1 An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units per minute per mL? minute. Given that the molar absorption coefficient (e) is 6220 M¹ cm and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in μmol per a. 8.04x10-8 b. 80.4 C. 8.04x10-5 d. 0.0804 QUESTION 3 A cell lysis buffer is made up with a pH of 7.9. What is the concentration of hydrogen ions in the buffer? a. 7.94 x 107 M b. 3.71 x 10-4M OC. 0.89M d. 1.26 x 10-8M QUESTION 4 What volume of 0.5M phosphate buffer needs to be added to a reaction mixture of final volume, 3mL, to give a final concentration of 0.2M? a. 1.2 mL b. 0.75 mL c. 0.12 mL d. 0.3 mL Click Save and Submit to save and submit. Click Save All Answers to save all answers. tab esc caps lock 40 Q A @ 2 F2 W S #3 F3 E S4 $ D F4 R % 5 FI F5 MacBook Pro 6 F6 10 G & 7 F7 H * CO 8 FB K O F10 Save All Answer P
Transcribed Image Text:Bb Take Test: Practical report 2 X C Apps LINDE Secure https://blackboard.le.ac.uk/webapps/assessment/take/launch.jsp?course_assessment_id=_39582_1&course_id=_41417_1&content_id=_ Unavailable in you... Amazing Opportu... Palmar Oceanfron...lv Bag, Medical Dr... JD Fila Disruptor II R... 3. Edexcel Revisio... Edexcel GC Question Completion Status: QUESTION 2 -1 An isocitrate dehydrogenase assay was performed on the enzyme sample and found to give an absorbance change at 340nm of 0.5 absorbance units per minute per mL? minute. Given that the molar absorption coefficient (e) is 6220 M¹ cm and the pathlength is 1cm, what is the rate of the enzyme catalysed reaction in μmol per a. 8.04x10-8 b. 80.4 C. 8.04x10-5 d. 0.0804 QUESTION 3 A cell lysis buffer is made up with a pH of 7.9. What is the concentration of hydrogen ions in the buffer? a. 7.94 x 107 M b. 3.71 x 10-4M OC. 0.89M d. 1.26 x 10-8M QUESTION 4 What volume of 0.5M phosphate buffer needs to be added to a reaction mixture of final volume, 3mL, to give a final concentration of 0.2M? a. 1.2 mL b. 0.75 mL c. 0.12 mL d. 0.3 mL Click Save and Submit to save and submit. Click Save All Answers to save all answers. tab esc caps lock 40 Q A @ 2 F2 W S #3 F3 E S4 $ D F4 R % 5 FI F5 MacBook Pro 6 F6 10 G & 7 F7 H * CO 8 FB K O F10 Save All Answer P
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