QUESTION 19 Which of the following is/are true regarding next generation sequencing? Next generation sequencing is also known as 'sequencing by synthesis' Next generation sequencing uses ddNTPs to identify the base at the end of a nucleotide chain Next generation sequencing will NOT work if chain terminating nucleotides are included in the reaction Next generation sequencing uses fluorescently labeled ddNTPs instead of radioactively labeled ddNTPs Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same time

Q: A circular DNA of 40 kb (40,000 bases) length is cut with a restriction enzyme whose precise…

A: The probability of a restriction enzyme to be found in a DNA is 4n, where n is the number of bases…

Q: Question 4 About endonucleases: A Restriction endonucleases always cut double-stranded DNA and are…

A: Nuclease is the nucleic acid digesting enzyme. Nuclease is of two types 1. exonuclease…

Q: Match the vocabulary word with the proper definition: enzyme that joins two pieces of DNA ✓ first…

A: DNA and RNA are two types of nucleic acids that are essential for life. They are composed of…

Q: QUESTION 31 Written from 5' to 3', the sequence of an RNA molecule made using the top strand of DNA…

A: INTRODUCTION Transcription is the process of making an RNA copy of a gene sequence. This copy,…

Q: Affected people are usually born to unaffected parents. Parents of affected people are usually…

A: 1. Autosomal recessive  2  X linked dominant  3. X linked recessive  4.  Y linked

Q: Question 13 Which regions (s) of the DNA are found in the final protein? Introns Exons UTRS poly-A…

A: Protein: Large biomolecules and macromolecules known as proteins are made up of one or more extended…

Q: How do eukaryotic and prokaryotic RNA polymerases compare? Despite their added complexity,…

A: RNA polymerase is an enzyme which is responsible for the transcription process, that takes place in…

Q: QUESTION 18 Which of the following statements about termination mechanisms for nucleic acid…

A: Transcription termination is the process where a nascent RNA is released from its complex with RNA…

Q: Question 27 To make RNA the nucleic acid sequences get read in the directi -- Sense → Antisense O…

A: Nitrogen-containing bases, phosphate groups, and sugar molecules make up nucleic acids. Each nucleic…

Q: Genetics Question 7

A: The objective of this question is to explain the practical applications of either Crispr, IPS cells,…

Q: Which of the following about the DNA synthesis reaction is false? O Phosphodiester bond formation…

A: DNA replication is the process by which DNA duplicates itself using DNA as a template and DNA…

Q: Question 31

A: Restriction endonucleases are the enzymes that are used to cut DNA (deoxyribonucleic acid) at the…

Q: QUESTION 3 Which types of mutations lead to frameshifts? O Deletions only Insertions only Insertions…

A: Protein synthesis is the process by which instructions present in DNA is converted into proteins,…

Q: Below is a segment of DNA which you wish to amplify using PCR (polymerase chain reaction). The…

A: The DNA polymerase always bonds with the free 3'-OH of the primer and elongated the new DNA strand…

Q: Imagine that a researcher has a cell from a genetically engineered organism and a clone of that same…

A: PCR stands for polymerase chain reactions. This is a technique used to amplify and make copies of an…

Q: If you were to observe the activity of acetylated DNA, you would expect it to: O be very actively…

A: As DNA is wrapped around the histone proteins;to form a more compact structure inside the…

Q: Question 2 Which example is describing a "missense" mutation? OThe normal amino acid sequence of a…

A: A mutation is a change that occurs in our DNA sequence, either due to mistakes when the DNA is…

Q: Advantages of using the PCR include the following EXCEPT A it will always give the desired product…

A: Introduction PCR:- It is a technique to make many copies of a specific DNA region in vitro, It…

Q: Which of the following statements describe disadvantages of the classical Sanger sequencing approach…

A: Since you have posted multiple questions, we will answer the first question for you. If you need any…

Q: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting…

A: The human genome is composed of sequences of nucleic acids, which are encoded as DNA(Deoxyribo…

Q: QUESTION 23 Below is a plasmid with restriction sites for BamHI and EcoRI. Several restriction…

A: Restriction endonucleases are a specific group of enzymes that are responsible for cutting double…

Q: QUESTION 1 The sequence of a DNA including the gene that you want to clone into a plasmid vector.…

A: Ans: The primers are set of oligo-nucleotide sequence used to initiate the synthesis or replication…

Q: the study of whole sets of genes and their interactions | Choose the application of computational…

A: Answer :  1) the study of whole sets of genes and their interactions - genomics.  2) the application…

Q: QUESTION 4 Which type of mutation will affect not only the amino acid encoded by the affected codon,…

A: Frameshift mutation can change every amino acid after the point of mutation . It caused by deletion…

Q: Question 7 Referring to the plasmid below, if a recombinant plasmid were obtained by inserting DNA…

A: The plasmid is the extrachromosomal DNA present in prokaryotic organisms. They have the ability to…

Q: What is experiment of Thermodynamics of Small Oligomeric Duplex DNA Denaturation.

A: Double-stranded DNA is the most common kind of DNA molecule found in nature. The strands can be…

Q: You want to perform PCR on the CDNA of the spike gene from a SARS CoV-2 sample so that you can…

A: PCR stands for polymerase chain reaction. It is used to amplify the gene of internet because forward…

Q: Question 1    DNA replication is said to be semi-conservative because   Question 1 options:   each…

A:

Q: Question 14 Which of the following is NOT true regarding E. coli replication on the lagging strand?…

A: In case of E. Coli replication of lagging strand: The synthesis is discontinuous and occurs 5' to 3'…

Q: Question:- Is DNA sequencing a in vivo, in vitro and/or in silico?   What product(s) is/are formed…

A: In vivo processes are processes done within the cell.In vitro processes are processes done in an…

Q: Question 39

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: Question 9 Which of the following is the missing link between DNA and Proteins according to the…

A: Central dogma  Central dogma a pictorial representation of genetic material which tells us how a…

Q: Review DNA sequencing and cloning tools. Match term and its description. a DNA contains DNA from two…

A: In biotechnology, particularly in rDNA technology, different tools and techniques are used to obtain…

Q: GQ2

A: The objective of the question is to determine the type of mutation that would occur if a specific…

Q: What were the characteristics of Watson and Cricks first model? O The bases were facing th

A: Watson and Crick were the discoverer of DNA double helix model. However, their initial model were…

Q: Question 7 What is the function of primase? It O synthesizes an RNA primer. O separates DNA strands.…

A: DNA replication is a process by which two identical copies of dsDNA are produced. It is completed in…

Q: Question 37

A: Biotechnology is the important field of biology that involves the modification of the organisms to…

Q: Can substitute bases in DNA because of structural similarity.

A: A mutagen is a physical or chemical agent that permanently alters genetic material, usually DNA, in…

Q: Which of the following statements is true regarding the formylmethionine that serves as the first…

A: Introduction Protein synthesis:- The process by which amino acids are linearly arranged into…

Q: Explain why the image below could not be from a eukaryote. What characteristics of the image would…

A: Eukaryotes are organisms that has nucleus and organelles within the cell. Nucleus is enclosed in the…

Q: QUESTION 4 Which of the following DNAs is most likely to contain the recognition sequence for a…

A: DNA (Deoxyribonucleic acid) recognition sequence is a specific linear sequence that is recognized by…

Q: Question 5. You are interpreting data on a DNA chip, or microarray. You expose the chip to a mixture…

A: A microarray is a collection of probes or oligos attached to a chip. Microarray is employed to…

Q: Question 32

A: RFLP or restriction fragment length polymorphism is a technique in molecular biology that makes use…

Q: Of the following DNA sequences which would you expect to have the highest melting temperature in its…

A: Melting temperature is defined as the temperature at which a double-stranded DNA molecule will break…

Q: Question 35

A: A DNA (deoxyribonucleic acid) library is a comprehensive collection of cloned DNA fragments from a…

Q: Question 3 In the polymerase chain reaction A conditions must be carefully controlled to prevent…

A: PCR is a technique which amplifies a DNA template or strand to produce thousands number of copies of…

Cancer Therapy