QUESTION 19 Which of the following is/are true regarding next generation sequencing? Next generation sequencing is also known as 'sequencing by synthesis' Next generation sequencing uses ddNTPs to identify the base at the end of a nucleotide chain Next generation sequencing will NOT work if chain terminating nucleotides are included in the reaction Next generation sequencing uses fluorescently labeled ddNTPs instead of radioactively labeled ddNTPs Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same time

Q: May you please help me with this? A sample of purified DNA was incubated with deoxyribonuclease…

A: DNA is the genetic material present in most living organisms. It is made of nucleotides, which…

Q: Match the vocabulary word with the proper definition: enzyme that joins two pieces of DNA ✓ first…

A: DNA and RNA are two types of nucleic acids that are essential for life. They are composed of…

Q: Procedure 1. 2. 3. 4. 5. 6. Fill in the data table below. Complete column B by writing the correct…

A: Process of synthesis of RNA from DNA is known as transcription. As the colum A in the table…

Q: Which of the following about the DNA synthesis reaction is false? O Phosphodiester bond formation…

A: DNA replication is the process by which DNA duplicates itself using DNA as a template and DNA…

Q: Suppose you are analyzing a nucleic acid sequence which contains guanine, cytosine, adenines, and…

A: DNA: DNA is a polymer made up of two polynucleotide chains that coil around one another to form a…

Q: Below is a segment of DNA which you wish to amplify using PCR (polymerase chain reaction). The…

A: The DNA polymerase always bonds with the free 3'-OH of the primer and elongated the new DNA strand…

Q: Imagine that a researcher has a cell from a genetically engineered organism and a clone of that same…

A: PCR stands for polymerase chain reactions. This is a technique used to amplify and make copies of an…

Q: How do fishes do gasexchange?

A: Fish are gill-bearing aquatic cranite animals that lack limbs with digits.

Q: If you have 7 large DNA duplexes, how many TOTAL DNA duplexes will you have after 7 rounds of PCR…

A: * PCR or polymerase chain reaction is chemical reaction  used to amplify the pieces of DNA. *Hence…

Q: The Shine-Dalgarno Sequence is used in bacteria eukaryotes both

A: The nucleotide segment at the 3' terminal of 16S ribosomal RNA (rRNA) has several pyrimidines with a…

Q: QUESTION 1 The sequence of a DNA including the gene that you want to clone into a plasmid vector.…

A: Ans: The primers are set of oligo-nucleotide sequence used to initiate the synthesis or replication…

Q: the study of whole sets of genes and their interactions | Choose the application of computational…

A: Answer :  1) the study of whole sets of genes and their interactions - genomics.  2) the application…

Q: Which statement on the migration of DNA fragments through agarose gels is false A) Small fragments…

A: Agarose gel electrophoresis is one the technique used in Recombinant DNA Technology and Molecular…

Q: Question 7 Referring to the plasmid below, if a recombinant plasmid were obtained by inserting DNA…

A: The plasmid is the extrachromosomal DNA present in prokaryotic organisms. They have the ability to…

Q: Question 1    DNA replication is said to be semi-conservative because   Question 1 options:   each…

A:

Q: 3. Now consider the following diagram, where two replication bubbles are about to meet. 5 3 Draw in…

A: The lagging strand is the one that starts to open in the 3' to 5' direction toward the replication…

Q: is it possible that pcr primers could bind and amplify a sequence of dna that is difference from the…

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: Question 39

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: QUESTION 33 Which of the following is the transfer of genes from generation to generation? O…

A: Question 33 Answer: Introduction: In horizontal gene transfer, the transfer of genetic material or…

Q: If you didn't know the sequence of the segment of DNA you want to amplify, how would you perform the…

A: Polymerase chain reaction is defined as the type of laboratory technique where it is used to amplify…

Q: Question 3 PUC plasmids are widely used because of the beta-lactamase gene that renders an easier…

A: Answer 1) The assertion is True. DNA segments from various sources are combined to make new DNA…

Q: QUESTION 4 Which of the following DNAs is most likely to contain the recognition sequence for a…

A: DNA (Deoxyribonucleic acid) recognition sequence is a specific linear sequence that is recognized by…

Q: Question 32

A: RFLP or restriction fragment length polymorphism is a technique in molecular biology that makes use…

Q: Question 1 Capping a pre-MRNA transcript and adding poly-A tail to its 3' end occurs in A eukaryotic…

A: Poly A tail is added to 3' end of the pre- mRNA once elongation is complete.  The polyA tail…

Q: Question 35

A: A DNA (deoxyribonucleic acid) library is a comprehensive collection of cloned DNA fragments from a…

Q: Question 3 In the polymerase chain reaction A conditions must be carefully controlled to prevent…

A: PCR is a technique which amplifies a DNA template or strand to produce thousands number of copies of…

Q: Why are consensus sequences found in cis-elements typically only 5-6 bases long? Question 19…

A: The consensus sequence is the calculated order of most frequent residues, either nucleotide or amino…

Q: Question 4. Why is a single-stranded, circular DNA an ineffective template for DNA polymerase?

A: Introduction Single-stranded DNA is a DNA molecule that has only one nucleotide strand while…

Q: What type of chemical bonds hold together a DNA double helix? weak hydrogen bonds between…

A: DNA It is one of nucleic acid that constitutes two polynucleotide chains. These chains coil around…

Q: which of the following is true for methyl directed repair O A. methyl directed repair cannot…

A: DNA replication is a long process that is done at considerable speed and hence, in spite of being a…

Q: QUESTION 2: What are the names of the steps of the PCR reaction that are repeated multiple times…

A: Polymerase Chain Reaction, or PCR, is a molecular biology technique employed to amplify and…

Q: For your experiment, you want to use a plasmid which is 8 kb long (see below). To digest the…

A: Plasmid is the double stranded, circular, extrachromosomal DNA, mainly present in bacteria (also…

Q: QUESTION 4 What is the name for the complex that forms when many ribosomes translate the same MRNA…

A: The name of complex that forms when many of ribosomes translate the same mRNA at same time is called…

Q: QUESTION 9 VMD can be used to do pairwise sequence alignments between two sequences with BLAST. True…

A: The field of molecular interaction has been widened with the use of computer tools. The different…

Q: Question 36

A: The word oligo in Oligonucleotide stands for scanty or few. Oligonucleotides are the moieties that…

Q: Review DNA sequencing and cloning tools. Which of these is not used to make a recombinant DNA?…

A: DNA cloning is a process by which identical copies are made from perticular pieces of DNA.

Q: Should PCR primers be complementary to each other? Explain the reasoning for your answer.

A: In PCR (Polymerase Chain Reaction) in order to start DNA replication short, single-stranded DNA…

Q: 8. Describe the possible outcome of a PCR experiment in which (a) one of the primers is…

A: Answer: Introduction: The polymerase chain reaction (PCR) technique, described by Kary Mullis in…

Q: These correct “overwinding” ahead of the replication forks by breaking, swiveling, and rejoining DNA…

A: DNA is the type of genetic material present in the organisms like humans and plants. The structure…

Q: Assuming DNA replicates semi-conservatively, which of these most closely approximates what you would…

A: DNA replicates semi-conservatively that means,  each time DNA replicates, the old strand is used as…

Q: Which of the following is FALSE regarding primase? O It synthesizes primers on the leading and…

A: Primase is an enzyme involved in the dna replication process . Itvis basically an rna polymerase…

Q: G-C A-T OT-A N-C C-G H N=C N-HO Which DNA base pair is represented in this figure? N-HIN HIN I H C-H

A: The monomeric units of DNA or deoxy nucleic acid are called nucleotides. Nucleotides are phosphate…

Q: A) How many origins of replication do you think the viral genome has, and why do you think this? B)…

A: Introduction Replication is a process by which DNA strands synthesize a complementary strand of…

Q: Use the following information to answer the next four questions. Nucleic Acid…

A: Nucleic acids are the polymers of the nucleotides.

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