PURPLE VESTIGIAL DIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (put/put.vg+/vg+) with a pure-breeding purple, vestigial (pu/pu;vg/vg) to produce an F1 generation that is all wild-type (pu*/pu;vg+/vg). Note that the F1 flies are all dihybrid. Next, you mate several F1 dihybrid females (put/pu;vg+/vg) with tester males, which are purple, vestigial (pu/pu;vg/vg). The offspring of this dihybrid testcross are: Phenotype Wild-type Purple, vestigial Vestigial Purple Genotype Tester Gamete Dihybrid Gamete Number 437 417 77 59 Copy the table into your notes and derive the dihybrid gametes following the example in the first section. The columns in blue (phenotypes and numbers of offspring) are what you can see and count. The genotypes of the testcross offspring (orange) must be deduced from the phenotypes and knowing that the tester contributed pu vg gametes. Finally, you can deduce the dihybrid gametes (green) by subtracting the tester gamete contribution from the offspring genotypes. Note that the two parental gamete types (+ + and pu vg) are the most abundant, as expected. Use the data to calculate the recombination frequency and the genetic map distance between the two genes. Record the map distance in your notes.
PURPLE VESTIGIAL DIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (put/put.vg+/vg+) with a pure-breeding purple, vestigial (pu/pu;vg/vg) to produce an F1 generation that is all wild-type (pu*/pu;vg+/vg). Note that the F1 flies are all dihybrid. Next, you mate several F1 dihybrid females (put/pu;vg+/vg) with tester males, which are purple, vestigial (pu/pu;vg/vg). The offspring of this dihybrid testcross are: Phenotype Wild-type Purple, vestigial Vestigial Purple Genotype Tester Gamete Dihybrid Gamete Number 437 417 77 59 Copy the table into your notes and derive the dihybrid gametes following the example in the first section. The columns in blue (phenotypes and numbers of offspring) are what you can see and count. The genotypes of the testcross offspring (orange) must be deduced from the phenotypes and knowing that the tester contributed pu vg gametes. Finally, you can deduce the dihybrid gametes (green) by subtracting the tester gamete contribution from the offspring genotypes. Note that the two parental gamete types (+ + and pu vg) are the most abundant, as expected. Use the data to calculate the recombination frequency and the genetic map distance between the two genes. Record the map distance in your notes.
Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter14: Chromosomes And Human Inheritance
Section: Chapter Questions
Problem 3GP: Human females have two X chromosomes (XX); males have one X and one Y chromosome (XY). a. With...
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Recombination frequency is the number of organisms that are produced from a genetic cross. The offsprings which do not have a genotype identical to either of the parents are the recombinant offsprings. The genetic map distance gives a rough physical distance of genes between two loci.
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