Protein Domain Mutations A) abasic nt endonuclease DNA-binding domain B) DNA gyrase C) DNA helicase D) DNA ligase E) DNA phytolase F) 5' exonuclease G) ExoI Asp to Ala Arg to Glu Gln to Asn Ala to His dimerization domain ATPase domain Ligation domain DNA-binding domain Ile to Ala nuclease domain Trp to Ala Gln to Asn nuclease domain H) EXOVII I) Gamma complex J) MSH proteins nuclease domain ATPase domain DNA-binding domain nuclease domain Arg to Pro Trp to Ala Asp to Ala Met to Tyr K) MutH L) Muts DNA-binding domain methyltransferase domain glycosylase domain RNA binding domain DNA binding domain DNA binding domain Thumb domain Palm domain Phe to Ala Ala to Asp M) 06-methyltransferase N) oxoG glycosylase 0) Primase P) Pol delta Q) Pol epsilon R) Pol III S) Pol III T) Pol III U) Pol IV V) RNase H Gln to Asn Met to Tyr Ile to Ala Gln to Asn Asp to Ala Asp to Ala Asp to Ala Ile to Ala Fingers domain DNA binding domain nuclease domain nuclease domain Phe to Ala W) RecJ X) Sliding Clamp Y) SSB 2) SSB AA) Tao protein AB) Tao protein AC) Uracil glycosylase binding domain DNA binding domain DNA binding domain protein binding domain protein binding domain glycosylase domain protein binding domain protein binding domain protein binding domain Ala to Asp Gln to Asn Arg to Lys Arg to Pro Ala to Asp Gln to Asn Met to Tyr Trp to Ala Asp to Ala Ala to Asp AD) UvrA AE) UvrD AF) XP Proteins
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Match the following
- __
DNA replication in E.coli stops after one replication cycle, and the new DNA strands contain a large number of individual RNAnucleotides spaced throughout the newly synthesized strand. - __ An E.coli mutant shows normal replication rates early I DNA replication, but the stops replicating DNA and is unable to continue.
- __ A mutant human cell line is unable to identify mutations occurring at times when the DNA is not being replicated.
- __ An E.coli mutant shows high mutation rates after DNA replication, and you observe fragments of methylated DNA cut out from the newly-synthesized chromosome.
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