Proliferating Cell Nuclear Antigen (PCNA) is a ring-shaped protein that forms a clamp around DNA. DNA polymerase binds to the outside of the PCNA clamp, keeping the polymerase tightly associated with DNA during replication. PCNA is a homo-oligomer, each subunit is shown as a different color in the image below. a) In the lab, you purify PCNA from mouse tissue. Based on the protein sequence, the extinction coefficient for a single PCNA molecule at 280 nm is e = 15,930 M cm. What is the extinction coefficient for the homo-oligomeric PCNA clamp? b) Your purified sample of murine PCNA absorbs 72% of incident light at 280 nm using a standard 1 cm path length cuvette. What is the concentration in micromolar?

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