Protein Concentration Standard Curve 0.7 y= 0.042x + 0.0378 R = 0.9867 0.6 0.5 0.4 0.3 0.2 0.1 10 12 14 16 Protein ug From the thesis of Ming-ho Mingo Yung, entitled "The regulation of thioredoxin system in cells under oxidative stress." 2. Suppose you collected UV/vis absorption data for several protein samples of known concentration at Amax =595 nm and constructed the calibration curve above. How much protein you have in an unknown sample if you get an absorbance value of 0.43 when you run your sample in a UV/vis spectrometer? Please show your work. would Absorbance 595nm

Protein Concentration Standard Curve 0.7 y= 0.042x + 0.0378 R = 0.9867 0.6 0.5 0.4 0.3 0.2 0.1 10 12 14 16 Protein ug From the thesis of Ming-ho Mingo Yung, entitled "The regulation of thioredoxin system in cells under oxidative stress." 2. Suppose you collected UV/vis absorption data for several protein samples of known concentration at Amax =595 nm and constructed the calibration curve above. How much protein you have in an unknown sample if you get an absorbance value of 0.43 when you run your sample in a UV/vis spectrometer? Please show your work. would Absorbance 595nm

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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