Proteasas are enzymes that break down proteins. Gastric proteasas are found in the stomach; intestinal proteases are found in the small intestine. Estimate the optimum pH for these two enzymes and identify as acidic or basic: Gastric proteases: pH= ____; acidic or basic Intestinal proteases activity: pH= ____; acidic or basic

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Proteasas are enzymes that break down proteins. Gastric proteasas are found in the stomach; intestinal proteases are found in the small intestine. Estimate the optimum pH for these two enzymes and identify as acidic or basic: Gastric proteases: pH= ____; acidic or basic Intestinal proteases activity: pH= ____; acidic or basic
**Enzyme Activity and pH Levels**

**Objective:** Understand the effect of pH on enzyme activity, focusing on gastric and intestinal proteases.

**Part 1: Ranking Enzyme Activity by pH**
1. **Rank pH Values:** The task involves arranging the pH levels from highest to lowest enzyme activity. Consider the specific enzyme's optimal pH range.

2. **Gas Production**
   - Identify which pH level resulted in the most gas production and explore the reasons why.

3. **Catalase Activity**
   - Determine the pH that is optimal for maximum catalase activity.

**Part 2: Graph Analysis**
- **Graph Description**: 
  - **Axes**: The x-axis represents pH levels, ranging from 0 to 12. The y-axis shows the relative rate of enzyme activity.
  - **Curves**: Two curves are present. The **dotted line** represents **gastric protease**, which peaks at around pH 2, indicating its highest activity at acidic conditions. The **solid line** shows **intestinal protease**, with peak activity between pH 8 and 9, suggesting optimal activity in basic conditions.

**Part 3: Protease pH Estimation**
4. **Protease Functions**:
   - **Gastric Protease**: Found in the stomach, optimal at pH 2, which is acidic.
   - **Intestinal Protease**: Located in the small intestine, optimal at pH 8-9, which is basic.

**Experiment 5: Hydrogen Peroxide Concentrations**
- *Note*: Documentation indicates "N/A" for hydrogen peroxide results, suggesting unavailability or irrelevance to the current context.

**Conclusion**
This analysis helps understand enzyme specificity in various pH environments, crucial for digestive processes. Understanding these patterns is key for applications in both health and industry.
Transcribed Image Text:**Enzyme Activity and pH Levels** **Objective:** Understand the effect of pH on enzyme activity, focusing on gastric and intestinal proteases. **Part 1: Ranking Enzyme Activity by pH** 1. **Rank pH Values:** The task involves arranging the pH levels from highest to lowest enzyme activity. Consider the specific enzyme's optimal pH range. 2. **Gas Production** - Identify which pH level resulted in the most gas production and explore the reasons why. 3. **Catalase Activity** - Determine the pH that is optimal for maximum catalase activity. **Part 2: Graph Analysis** - **Graph Description**: - **Axes**: The x-axis represents pH levels, ranging from 0 to 12. The y-axis shows the relative rate of enzyme activity. - **Curves**: Two curves are present. The **dotted line** represents **gastric protease**, which peaks at around pH 2, indicating its highest activity at acidic conditions. The **solid line** shows **intestinal protease**, with peak activity between pH 8 and 9, suggesting optimal activity in basic conditions. **Part 3: Protease pH Estimation** 4. **Protease Functions**: - **Gastric Protease**: Found in the stomach, optimal at pH 2, which is acidic. - **Intestinal Protease**: Located in the small intestine, optimal at pH 8-9, which is basic. **Experiment 5: Hydrogen Peroxide Concentrations** - *Note*: Documentation indicates "N/A" for hydrogen peroxide results, suggesting unavailability or irrelevance to the current context. **Conclusion** This analysis helps understand enzyme specificity in various pH environments, crucial for digestive processes. Understanding these patterns is key for applications in both health and industry.
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