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Prostaglandins are a class of cicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20- carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG₂, the immediate precursor of many different prostaglandins. Arachidonic acid (mm) 0.175 0.210 0.315 0.525 1.23 Vmax Km 38.91 The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. 0.688 Rate of formation of PGG2 (mm/min) Incorrect 12.0 13.5 17.1 21.6 28.0 Rate of formation of PGG₂ with 10 mg/ml ibuprofen (mm/min) 7.50 8.64 11.6 15.9 23.3 competitive inhibition uncompetitive inhibition Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. mixed inhibition mM/min mM

Prostaglandins are a class of cicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20- carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG₂, the immediate precursor of many different prostaglandins. Arachidonic acid (mm) 0.175 0.210 0.315 0.525 1.23 Vmax Km 38.91 The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. 0.688 Rate of formation of PGG2 (mm/min) Incorrect 12.0 13.5 17.1 21.6 28.0 Rate of formation of PGG₂ with 10 mg/ml ibuprofen (mm/min) 7.50 8.64 11.6 15.9 23.3 competitive inhibition uncompetitive inhibition Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. mixed inhibition mM/min mM

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20- carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG₂, the immediate precursor of many different prostaglandins. Rate of formation of PGG₂ with 10 mg/ml ibuprofen (mm/min) Arachidonic acid (mm) VIX 0.175 0.210 0.315 0.525 1.23 Km The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. 38.91 0.688 Rate of formation of PGG2 (mm/min) Incorrect 12.0 13.5 17.1 21.6 28.0 7.50 8.64 11.6 15.9 23.3 competitive inhibition uncompetitive inhibition Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. mixed inhibition mM/min mM
Transcribed Image Text:Prostaglandins are a class of eicosanoids, fatty acid derivatives with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20- carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid to PGG₂, the immediate precursor of many different prostaglandins. Rate of formation of PGG₂ with 10 mg/ml ibuprofen (mm/min) Arachidonic acid (mm) VIX 0.175 0.210 0.315 0.525 1.23 Km The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. 38.91 0.688 Rate of formation of PGG2 (mm/min) Incorrect 12.0 13.5 17.1 21.6 28.0 7.50 8.64 11.6 15.9 23.3 competitive inhibition uncompetitive inhibition Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase. mixed inhibition mM/min mM
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