Results:TubRanking of reactionIodine TestBenedict's Testerate134) Procedure:1. Prepare 6 test tubes and place 1 ml saliva in each.2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml totube 5 and 10 ml to tube 6. Mix thoroughly.3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) andadd 1 ml of 1% starch paste. Mix well and heat in a water bath withtemperature maintained at 40°C for 30 minutes.4. Divide each of the contents of each tube into 2 and test with:a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KIand note the color produced. Compare the intensity of color in each of the6 tubes.b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half ofeach tube and heat in a boiling water bath for 3 minutes. Note color ofprecipitate.5. Rank each tube according to decreasing reaction rate one (1) being the fastestto hydrolyze and 6 being the slowest.

Iodine test: This is specific test for the identification of starch in the given sample. If starch…

Answered: Procedure: 1. Prepare 6 test tubes and…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.
Answer the following three questions using the chart referenced on the second image. Please type them out instead of writing them, I’m not good at reading hand writing.
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Hi, my question is 6 ml of urine is added to 30 ml of water. 0.1 ml is plated. 109 colonies form. What is the CFU/ml of the urine culture?
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Analysis of the pathological constituents GIVE THE FOLLOWING: - Name of Test/Test Reagent - Positive Result 1) Protein (Note: Presence of protein in urine is termed as proteinuria or albuminuria) A. Heat and Acetic acid test Fill a test about ¾ full of urine. Heat the upper portion gently to boiling for 1 -2 minutes being careful not to shake the tube. Rotate the tube to prevent overheating. A turbidity may be due to albumin, phosphates or carbonates. Add 3 drops of acetic acid drop by drop while boiling between each drop. If turbidity disappears, it is due to carbonates and phosphates. B. Heller’s nitric acid test Place 1 ml of urine sample in a test tube. Hold the test tube in an inclined position and carefully pour 1 drops of conc HNO3 down the side of the test tube. Note formation of white ring at the zone of contact of the two solutions which is an indication of the presence of protein.
Modified True or False: Write CMB if the statement or question is correct. If the statement or question is incorrect write THE CORRECT ANSWER and UNDERLINE, the word/s that makes the statement or question incorrect. ´. The iodine test is used to test for the presence of starch. 2. 1. The Benedict's test identifies reducing sugars Biuret test is a general test for compounds having two or more peptide 4. 3. Sudan III/IV test is used to detect the hydrocarbon chains of lipids. 5. In 1842, Karl Nageli, was the first person who had seen rod-like structures present in the animal cells' nucleus. chromosomes were first studied by Walther Flemming in 1888. 7. 6. Satellite is a knob-like structure at the end of a chromosome that is present beyond the secondary constriction or distal to it. 8. Allosome_is the sex chromosome Karyotyping based on symmetry was formulated by Levitzky in 1941 10. Parm is the shorth part of chromosome An MRNA with many open reading frames is called polycistronic…
Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
1. What are the 3 phase of the routine stool examination? 2. What would be the pre-analytical phase in doing Kato technique? 3. Discuss the Cleaning and Disinfection involved in this test after the procedure.

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