Homework Help is Here – Start Your Trial Now!
Science
Biology
PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with a clean cloth or tissue paper. A drop of water placed on a clean slide will spread out evenly. If the drop rounds up, clean the slide again. The slide should be thoroughly cleaned and freed of grease. 2. If the specimen comes from liquid culture, remove a loopful as in exercise 2. Spread the loopful evenly and thinly over a 5 sq. mm area. If the bacterial specimen comes from a solid medium, get a loopful by following aseptic technique, put drop of water in the slide and mix until a turbid mixture is acquired before spreading evenly. 3. Dry the film by air. Fix smear by heat or by alcohol. In heat fixing, the slide is passed, film slide up quickly over the flame of the alcohol lamp 2 or 3 times. Do not scorch. The slide should feel hot to the fingers, but should not burn them. The alcohol treatment consists of covering the smear with 95% alcohol for one minute then draining off. Heat fixing is never used with preparations made of milk. The purpose of fixation is to kill the microorganisms, coagulate the protoplasm of the cell and cause it to adhere to the slide. The best bacterial smears are usually made by removing a small amount or surface growth from agar culture and mixing with water. It is often possible to use a drop of culture growing in a liquid medium, but such a smear is not always so satisfactory since certain

PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with a clean cloth or tissue paper. A drop of water placed on a clean slide will spread out evenly. If the drop rounds up, clean the slide again. The slide should be thoroughly cleaned and freed of grease. 2. If the specimen comes from liquid culture, remove a loopful as in exercise 2. Spread the loopful evenly and thinly over a 5 sq. mm area. If the bacterial specimen comes from a solid medium, get a loopful by following aseptic technique, put drop of water in the slide and mix until a turbid mixture is acquired before spreading evenly. 3. Dry the film by air. Fix smear by heat or by alcohol. In heat fixing, the slide is passed, film slide up quickly over the flame of the alcohol lamp 2 or 3 times. Do not scorch. The slide should feel hot to the fingers, but should not burn them. The alcohol treatment consists of covering the smear with 95% alcohol for one minute then draining off. Heat fixing is never used with preparations made of milk. The purpose of fixation is to kill the microorganisms, coagulate the protoplasm of the cell and cause it to adhere to the slide. The best bacterial smears are usually made by removing a small amount or surface growth from agar culture and mixing with water. It is often possible to use a drop of culture growing in a liquid medium, but such a smear is not always so satisfactory since certain

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

Make a flowchart for the PREPARATION OF SMEARS

PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with a clean cloth or tissue paper. A drop of water placed on a clean slide will spread out evenly. If the drop rounds up, clean the slide again. The slide should be thoroughly cleaned and freed of grease. 2. If the specimen comes from liquid culture, remove a loopful as in exercise 2. Spread the loopful evenly and thinly over a 5 sq. mm area. If the bacterial specimen comes from a solid medium, get a loopful by following aseptic technique, put drop of water in the slide and mix until a turbid mixture is acquired before spreading evenly. 3. Dry the film by air. Fix smear by heat or by alcohol. In heat fixing, the slide is passed, film slide up quickly over the flame of the alcohol lamp 2 or 3 times. Do not scorch. The slide should feel hot to the fingers, but should not burn them. The alcohol treatment consists of covering the smear with 95% alcohol for one minute then draining off. Heat fixing is never used with preparations made of milk. The purpose of fixation is to kill the microorganisms, coagulate the protoplasm of the cell and cause it to adhere to the slide. The best bacterial smears are usually made by removing a small amount or surface growth from agar culture and mixing with water. It is often possible to use a drop of culture growing in a liquid medium, but such a smear is not always so satisfactory since certain
Transcribed Image Text:PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with a clean cloth or tissue paper. A drop of water placed on a clean slide will spread out evenly. If the drop rounds up, clean the slide again. The slide should be thoroughly cleaned and freed of grease. 2. If the specimen comes from liquid culture, remove a loopful as in exercise 2. Spread the loopful evenly and thinly over a 5 sq. mm area. If the bacterial specimen comes from a solid medium, get a loopful by following aseptic technique, put drop of water in the slide and mix until a turbid mixture is acquired before spreading evenly. 3. Dry the film by air. Fix smear by heat or by alcohol. In heat fixing, the slide is passed, film slide up quickly over the flame of the alcohol lamp 2 or 3 times. Do not scorch. The slide should feel hot to the fingers, but should not burn them. The alcohol treatment consists of covering the smear with 95% alcohol for one minute then draining off. Heat fixing is never used with preparations made of milk. The purpose of fixation is to kill the microorganisms, coagulate the protoplasm of the cell and cause it to adhere to the slide. The best bacterial smears are usually made by removing a small amount or surface growth from agar culture and mixing with water. It is often possible to use a drop of culture growing in a liquid medium, but such a smear is not always so satisfactory since certain
constituents of the medium may prevent the bacteria from adhering to the slide or may interfere with staining. The suspension used should always be diluted. Gram staining Stain your smear with ammonium oxalate crystal violet for one minute. Wash thoroughly but gently with tap water. Cover smear with iodine solution for one minute. Wash in tap water and shake off excess moisture. Decolorize with 95% ethyl alcohol for 15 to 20 seconds. Wash with water. Counterstain with safranin solution for 30 seconds. Wash with tap water and blot dry. The gram (+) positive organisms appear blue or deep violet; the gram (-) negative, red or pink.
Transcribed Image Text:constituents of the medium may prevent the bacteria from adhering to the slide or may interfere with staining. The suspension used should always be diluted. Gram staining Stain your smear with ammonium oxalate crystal violet for one minute. Wash thoroughly but gently with tap water. Cover smear with iodine solution for one minute. Wash in tap water and shake off excess moisture. Decolorize with 95% ethyl alcohol for 15 to 20 seconds. Wash with water. Counterstain with safranin solution for 30 seconds. Wash with tap water and blot dry. The gram (+) positive organisms appear blue or deep violet; the gram (-) negative, red or pink.
Expert Solution
steps

Step by step

Solved in 2 steps with 2 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Serology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS